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Characterization of 1-chlorohexane halidohydrolase, a dehalogenase of wide substrate range from an Arthrobacter sp.

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Characterization_of_1_Chlorohexane.pdf
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1987

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Scholtz, Rudolf
Leisinger, Thomas
Suter, Franz

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Journal of Bacteriology. 1987, 169(11), pp. 5016-5021. ISSN 0021-9193. eISSN 1098-5530

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1-Chlorohexane halidohydrolase from Arthrobacter sp. strain HA1 was purified to homogeneity by fractional precipitation, ion-exchange chromatography, gel filtration, and high-performance liquid chromatography gel filtration. The enzyme was a monomer with a molecular weight of about 37,000; its amino acid composition and N-terminal sequence were determined. The enzyme had a broad optimum around pH 9.5, a temperature optimum near 50 degrees C, an activation energy of 40 kJ/mol, and a molecular activity of 0.9 kat/mol. The substrate range of the enzyme included at least 50 halogenated compounds. 1-Chloroalkanes (C3 to C10), 1-bromoalkanes (C1 to C9), and 1-iodoalkanes (C1 to C7), but no 1-fluoroalkane, were substrates. Subterminally substituted, branched-chain, and nonsaturated haloalkanes were dehalogenated. Some halogenated aromatic substrates, e.g., bromobenzene and benzyl bromide, were hydrolyzed. Several alpha,omega-dihaloalkanes were subject to double dehalogenation. Thus, 1,2-dibromoethane was hydrolyzed first to 2-bromoethanol and then to 1,2-dihydroxyethane. Crude extracts of strain HA1 were found to contain a debrominase that cleaved bromoalkanes with long alkyl chains.

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570 Biowissenschaften, Biologie

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ISO 690SCHOLTZ, Rudolf, Thomas LEISINGER, Franz SUTER, Alasdair M. COOK, 1987. Characterization of 1-chlorohexane halidohydrolase, a dehalogenase of wide substrate range from an Arthrobacter sp.. In: Journal of Bacteriology. 1987, 169(11), pp. 5016-5021. ISSN 0021-9193. eISSN 1098-5530
BibTex
@article{Scholtz1987Chara-8613,
  year={1987},
  title={Characterization of 1-chlorohexane halidohydrolase, a dehalogenase of wide substrate range from an Arthrobacter sp.},
  number={11},
  volume={169},
  issn={0021-9193},
  journal={Journal of Bacteriology},
  pages={5016--5021},
  author={Scholtz, Rudolf and Leisinger, Thomas and Suter, Franz and Cook, Alasdair M.}
}
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