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Three Novel Clostridia Isolates Produce n-Caproate and iso-Butyrate from Lactate : Comparative Genomics of Chain-Elongating Bacteria

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2020

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Liu, Bin
Popp, Denny
Sträuber, Heike
Harms, Hauke
Kleinsteuber, Sabine

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Published

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Microorganisms. MDPI. 2020, 8(12), 1970. eISSN 2076-2607. Available under: doi: 10.3390/microorganisms8121970

Zusammenfassung

The platform chemicals n-caproate and iso-butyrate can be produced by anaerobic fermentation from agro-industrial residues in a process known as microbial chain elongation. Few lactate-consuming chain-elongating species have been isolated and knowledge on their shared genetic features is still limited. Recently we isolated three novel clostridial strains (BL-3, BL-4, and BL-6) that convert lactate to n-caproate and iso-butyrate. Here, we analyzed the genetic background of lactate-based chain elongation in these isolates and other chain-elongating species by comparative genomics. The three strains produced n-caproate, n-butyrate, iso-butyrate, and acetate from lactate, with the highest proportions of n-caproate (18%) for BL-6 and of iso-butyrate (23%) for BL-4 in batch cultivation at pH 5.5. They show high genomic heterogeneity and a relatively small core-genome size. The genomes contain highly conserved genes involved in lactate oxidation, reverse β-oxidation, hydrogen formation and either of two types of energy conservation systems (Rnf and Ech). Including genomes of another eleven experimentally validated chain-elongating strains, we found that the chain elongation-specific core-genome encodes the pathways for reverse β-oxidation, hydrogen formation and energy conservation, while displaying substantial genome heterogeneity. Metabolic features of these isolates are important for biotechnological applications in n-caproate and iso-butyrate production.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

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novel clostridial species; carboxylate platform; medium-chain carboxylates; branched-chain carboxylates; anaerobic fermentation; reverse β-oxidation

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ISO 690LIU, Bin, Denny POPP, Nicolai MÜLLER, Heike STRÄUBER, Hauke HARMS, Sabine KLEINSTEUBER, 2020. Three Novel Clostridia Isolates Produce n-Caproate and iso-Butyrate from Lactate : Comparative Genomics of Chain-Elongating Bacteria. In: Microorganisms. MDPI. 2020, 8(12), 1970. eISSN 2076-2607. Available under: doi: 10.3390/microorganisms8121970
BibTex
@article{Liu2020-12-11Three-52167,
  year={2020},
  doi={10.3390/microorganisms8121970},
  title={Three Novel Clostridia Isolates Produce n-Caproate and iso-Butyrate from Lactate : Comparative Genomics of Chain-Elongating Bacteria},
  number={12},
  volume={8},
  journal={Microorganisms},
  author={Liu, Bin and Popp, Denny and Müller, Nicolai and Sträuber, Heike and Harms, Hauke and Kleinsteuber, Sabine},
  note={Article Number: 1970}
}
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