Publikation: Three Novel Clostridia Isolates Produce n-Caproate and iso-Butyrate from Lactate : Comparative Genomics of Chain-Elongating Bacteria
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The platform chemicals n-caproate and iso-butyrate can be produced by anaerobic fermentation from agro-industrial residues in a process known as microbial chain elongation. Few lactate-consuming chain-elongating species have been isolated and knowledge on their shared genetic features is still limited. Recently we isolated three novel clostridial strains (BL-3, BL-4, and BL-6) that convert lactate to n-caproate and iso-butyrate. Here, we analyzed the genetic background of lactate-based chain elongation in these isolates and other chain-elongating species by comparative genomics. The three strains produced n-caproate, n-butyrate, iso-butyrate, and acetate from lactate, with the highest proportions of n-caproate (18%) for BL-6 and of iso-butyrate (23%) for BL-4 in batch cultivation at pH 5.5. They show high genomic heterogeneity and a relatively small core-genome size. The genomes contain highly conserved genes involved in lactate oxidation, reverse β-oxidation, hydrogen formation and either of two types of energy conservation systems (Rnf and Ech). Including genomes of another eleven experimentally validated chain-elongating strains, we found that the chain elongation-specific core-genome encodes the pathways for reverse β-oxidation, hydrogen formation and energy conservation, while displaying substantial genome heterogeneity. Metabolic features of these isolates are important for biotechnological applications in n-caproate and iso-butyrate production.
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LIU, Bin, Denny POPP, Nicolai MÜLLER, Heike STRÄUBER, Hauke HARMS, Sabine KLEINSTEUBER, 2020. Three Novel Clostridia Isolates Produce n-Caproate and iso-Butyrate from Lactate : Comparative Genomics of Chain-Elongating Bacteria. In: Microorganisms. MDPI. 2020, 8(12), 1970. eISSN 2076-2607. Available under: doi: 10.3390/microorganisms8121970BibTex
@article{Liu2020-12-11Three-52167, year={2020}, doi={10.3390/microorganisms8121970}, title={Three Novel Clostridia Isolates Produce n-Caproate and iso-Butyrate from Lactate : Comparative Genomics of Chain-Elongating Bacteria}, number={12}, volume={8}, journal={Microorganisms}, author={Liu, Bin and Popp, Denny and Müller, Nicolai and Sträuber, Heike and Harms, Hauke and Kleinsteuber, Sabine}, note={Article Number: 1970} }
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