Publikation: Anaerobic degradation of 3-hydroxybenzoate by a newly isolated nitrate-reducing bacterium
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Abstract A gram-negative nitrate-reducing bacterium, strain Asl-3, was isolated from activated sludge with nitrate and 3-hydroxybenzoate as sole source of carbon and energy. The new isolate was faculaatively anaerobic, catalase- and oxidase-positive and polarly monotrichously flagellated. In addition to nitrate, nitrite, N2O, and O2 served as electron acceptors. Growth with 3-hydroxybenzoate and nitrate was biphasic: nitrate was completely reduced to nitrite before nitrite reduction to N2 started. Benzoate, 3-hydroxybenzoate, 4-hydroxybenzoate, protocatechuate or phenyl-acetate served as electron and carbon source under aerobic and anaerobic conditions. During growth with excess carbon source, poly-β-hydroxybutyrate was formed. These characteristics allow the affiliation of strain Asl-3 with the family Pseudomonadaceae. Analogous to the pathway of 4-hydroxybenzoate degradation in other bacteria, the initial step in anaerobic 3-hydroxybenzoate degradation by this organism was activation to 3-hydroxy-benzoyl-CoA in an ATP-consuming reaction. Cell extracts of 3-hydroxybenzoate-grown cells exhibited 3-hydroxybenzoyl-CoA synthetase activity of 190 nmol min−1 mg protein−1 as well as benzoyl-CoA synthetase activity of 86 nmol min−1 mg protein−1. A reductive dehydroxylation of 3-hydroxybenzoyl-CoA could not be demonstrated due to rapid hydrolysis of chemically synthesized 3-hydroxybenzoyl-CoA by cell extracts.
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HEISING, Silke, Andreas BRUNE, Bernhard SCHINK, 1991. Anaerobic degradation of 3-hydroxybenzoate by a newly isolated nitrate-reducing bacterium. In: FEMS Microbiology Letters. 1991, 84(3), pp. 267-272. ISSN 0378-1097. eISSN 1574-6968. Available under: doi: 10.1111/j.1574-6968.1991.tb04608.xBibTex
@article{Heising1991Anaer-7126, year={1991}, doi={10.1111/j.1574-6968.1991.tb04608.x}, title={Anaerobic degradation of 3-hydroxybenzoate by a newly isolated nitrate-reducing bacterium}, number={3}, volume={84}, issn={0378-1097}, journal={FEMS Microbiology Letters}, pages={267--272}, author={Heising, Silke and Brune, Andreas and Schink, Bernhard} }
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