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Identification of the biosynthetic gene cluster for 3-methylarginine, a toxin Produced by Pseudomonas syringae pv. syringae 22d/93

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2010

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Braun, Sascha D.
Hofmann, Janine
Wensing, Annette
Ullrich, Matthias
Weingart, Helge
Völksch, Beate

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http://nbn-resolving.de/urn:nbn:de:bsz:352-152914
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https://doi.org/10.1128/AEM.00666-09
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Applied and Environmental Microbiology. 2010, 76(8), pp. 2500-2508. ISSN 0099-2240. eISSN 1098-5336. Available under: doi: 10.1128/AEM.00666-09

Zusammenfassung

The epiphyte Pseudomonas syringae pv. syringae 22d/93 (Pss22d) produces the rare amino acid 3-methylarginine (MeArg), which is highly active against the closely related soybean pathogen Pseudomonas syringae pv. glycinea. Since these pathogens compete for the same habitat, Pss22d is a promising candidate for biocontrol of P. syringae pv. glycinea. The MeArg biosynthesis gene cluster codes for the S-adenosylmethionine (SAM)-dependent methyltransferase MrsA, the putative aminotransferase MrsB, and the amino acid exporter MrsC. Transfer of the whole gene cluster into Escherichia coli resulted in heterologous production of MeArg. The methyltransferase MrsA was overexpressed in E. coli as a His-tagged protein and functionally characterized (Km, 7 mM; kcat, 85 min–1). The highly selective methyltransferase MrsA transfers the methyl group from SAM into 5-guanidino-2-oxo-pentanoic acid to yield 5-guanidino-3-methyl-2-oxo-pentanoic acid, which then only needs to be transaminated to result in the antibiotic MeArg.

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570 Biowissenschaften, Biologie

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ISO 690BRAUN, Sascha D., Janine HOFMANN, Annette WENSING, Matthias ULLRICH, Helge WEINGART, Dieter SPITELLER, Beate VÖLKSCH, 2010. Identification of the biosynthetic gene cluster for 3-methylarginine, a toxin Produced by Pseudomonas syringae pv. syringae 22d/93. In: Applied and Environmental Microbiology. 2010, 76(8), pp. 2500-2508. ISSN 0099-2240. eISSN 1098-5336. Available under: doi: 10.1128/AEM.00666-09
BibTex
@article{Braun2010-04Ident-15291,
  year={2010},
  doi={10.1128/AEM.00666-09},
  title={Identification of the biosynthetic gene cluster for 3-methylarginine, a toxin Produced by Pseudomonas syringae pv. syringae 22d/93},
  number={8},
  volume={76},
  issn={0099-2240},
  journal={Applied and Environmental Microbiology},
  pages={2500--2508},
  author={Braun, Sascha D. and Hofmann, Janine and Wensing, Annette and Ullrich, Matthias and Weingart, Helge and Spiteller, Dieter and Völksch, Beate}
}
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