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In Vivo Two-Photon Imaging of the Olfactory System in Insects

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2018

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Haase, Albrecht

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https://doi.org/10.1007/978-1-4939-8609-5_15
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MARQUES SIMOES DE SOUZA, Fabio, ed. and others. Olfactory receptors : methods and protocols. New York, NY: Humana Press, 2018, pp. 179-219. Methods in molecular biology. 1820. ISBN 978-1-4939-8608-8. Available under: doi: 10.1007/978-1-4939-8609-5_15

Zusammenfassung

This chapter describes how to apply two-photon neuroimaging to study the insect olfactory system in vivo. It provides a complete protocol for insect brain functional imaging, with some additional remarks on the acquisition of morphological information from the living brain. We discuss the most important choices to make when buying or building a two-photon laser-scanning microscope. We illustrate different possibilities of animal preparation and brain tissue labeling for in vivo imaging. Finally, we give an overview of the main methods of image data processing and analysis, followed by a short description of pioneering applications of this imaging modality.

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570 Biowissenschaften, Biologie

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ISO 690PAOLI, Marco, Albrecht HAASE, 2018. In Vivo Two-Photon Imaging of the Olfactory System in Insects. In: MARQUES SIMOES DE SOUZA, Fabio, ed. and others. Olfactory receptors : methods and protocols. New York, NY: Humana Press, 2018, pp. 179-219. Methods in molecular biology. 1820. ISBN 978-1-4939-8608-8. Available under: doi: 10.1007/978-1-4939-8609-5_15
BibTex
@incollection{Paoli2018TwoPh-44512,
  year={2018},
  doi={10.1007/978-1-4939-8609-5_15},
  title={In Vivo Two-Photon Imaging of the Olfactory System in Insects},
  number={1820},
  isbn={978-1-4939-8608-8},
  publisher={Humana Press},
  address={New York, NY},
  series={Methods in molecular biology},
  booktitle={Olfactory receptors : methods and protocols},
  pages={179--219},
  editor={Marques Simoes de Souza, Fabio},
  author={Paoli, Marco and Haase, Albrecht}
}
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