Publikation:

Cathepsin B Acts as a Dominant Execution Protease in Tumor Cell Apoptosis Induced by Tumor Necrosis Factor

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2001

Autor:innen

Foghsgaard, Lasse
Wissing, Dorte
Mauch, Daniel
Boes, Marianne
Elling, Folmer
Jäättelä, Marja

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The Journal of Cell Biology. 2001, 153(5), pp. 999-1009. ISSN 0021-9525. eISSN 1540-8140. Available under: doi: 10.1083/jcb.153.5.999

Zusammenfassung

Death receptors can trigger cell demise dependent or independent of caspases. In WEHI-S fibrosarcoma cells, tumor necrosis factor (TNF) induced an increase in cytosolic cathepsin B activity followed by death with apoptotic features. Surprisingly, this process was enhanced by low, but effectively inhibiting, concentrations of pan-caspase inhibitors. Contrary to caspase inhibitors, a panel of pharmacological cathepsin B inhibitors, the endogenous cathepsin inhibitor cystatin A as well as antisense-mediated depletion of cathepsin B rescued WEHI-S cells from apoptosis triggered by TNF or TNF-related apoptosis-inducing ligand. Thus, cathepsin B can take over the role of the dominant execution protease in death receptor-induced apoptosis. The conservation of this alternative execution pathway was further examined in other tumor cell lines. Here, cathepsin B acted as an essential downstream mediator of TNF-triggered and caspase-initiated apoptosis cascade, whereas apoptosis of primary cells was only minimally dependent on cathepsin B. These data imply that cathepsin B, which is commonly overexpressed in human primary tumors, may have two opposing roles in malignancy, reducing it by its proapoptotic features and enhancing it by its known facilitation of invasion.

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570 Biowissenschaften, Biologie

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ISO 690FOGHSGAARD, Lasse, Dorte WISSING, Daniel MAUCH, Marianne BOES, Folmer ELLING, Marcel LEIST, Marja JÄÄTTELÄ, 2001. Cathepsin B Acts as a Dominant Execution Protease in Tumor Cell Apoptosis Induced by Tumor Necrosis Factor. In: The Journal of Cell Biology. 2001, 153(5), pp. 999-1009. ISSN 0021-9525. eISSN 1540-8140. Available under: doi: 10.1083/jcb.153.5.999
BibTex
@article{Foghsgaard2001Cathe-8525,
  year={2001},
  doi={10.1083/jcb.153.5.999},
  title={Cathepsin B Acts as a Dominant Execution Protease in Tumor Cell Apoptosis Induced by Tumor Necrosis Factor},
  number={5},
  volume={153},
  issn={0021-9525},
  journal={The Journal of Cell Biology},
  pages={999--1009},
  author={Foghsgaard, Lasse and Wissing, Dorte and Mauch, Daniel and Boes, Marianne and Elling, Folmer and Leist, Marcel and Jäättelä, Marja}
}
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