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Probiotic Enterococcus faecalis Symbioflor® down regulates virulence genes of EHEC in vitro and decrease pathogenicity in a Caenorhabditis elegans model

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2017

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Neuhaus, Klaus
Lamparter, Marina C.
Zölch, Benjamin
Landstorfer, Richard
Spanier, Britta
Ehrmann, Matthias A.
Vogel, Rudi F.

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https://doi.org/10.1007/s00203-016-1291-8
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Archives of Microbiology. 2017, 199(2), pp. 203-213. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/s00203-016-1291-8

Zusammenfassung

Enterohemorrhagic E. coli O157:H7 (EHEC) shorten the lifespan of Caenorhabditis elegans compared to avirulent bacteria. Co-feeding EHEC with Enterococcus faecalis Symbioflor(®) significantly increased the worms' lifespan. The transcriptome of EHEC grown in vitro with or without Symbioflor(®) was analyzed using RNA-seq. The analysis revealed downregulation of several virulence-associated genes in the presence of Symbioflor(®), including virulence key genes (e.g., LEE, flagellum, quorum-sensing). The downregulation of the LEE genes was corroborated by lux-transposon mutants. Upregulated genes included acid response genes, due to a decrease in pH exerted by Symbioflor(®). Further genes indicate cellular stress in EHEC (e.g. prophage/mobile elements involved in excision, cell lysis, and cell division inhibition). Thus, the observed protection of C. elegans during an EHEC infection by the probiotic Symbioflor(®) is suggested to be caused by triggering concomitant transcriptomic changes. To verify the biological relevance of this modulation, exemplary genes found to be influenced by Symbioflor(®) were knocked out (fliD, espB, Z3136, Z3917, and L7052). The lifespan of nematodes changed when using knock-outs as food source and the effect could be complemented in trans. In summary, Symbioflor(®) appears to be a protective probiotic in the nematode model.

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ISO 690NEUHAUS, Klaus, Marina C. LAMPARTER, Benjamin ZÖLCH, Richard LANDSTORFER, Svenja SIMON, Britta SPANIER, Matthias A. EHRMANN, Rudi F. VOGEL, 2017. Probiotic Enterococcus faecalis Symbioflor® down regulates virulence genes of EHEC in vitro and decrease pathogenicity in a Caenorhabditis elegans model. In: Archives of Microbiology. 2017, 199(2), pp. 203-213. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/s00203-016-1291-8
BibTex
@article{Neuhaus2017Probi-38638,
  year={2017},
  doi={10.1007/s00203-016-1291-8},
  title={Probiotic Enterococcus faecalis Symbioflor® down regulates virulence genes of EHEC in vitro and decrease pathogenicity in a Caenorhabditis elegans model},
  number={2},
  volume={199},
  issn={0302-8933},
  journal={Archives of Microbiology},
  pages={203--213},
  author={Neuhaus, Klaus and Lamparter, Marina C. and Zölch, Benjamin and Landstorfer, Richard and Simon, Svenja and Spanier, Britta and Ehrmann, Matthias A. and Vogel, Rudi F.}
}
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    <dcterms:abstract xml:lang="eng">Enterohemorrhagic E. coli O157:H7 (EHEC) shorten the lifespan of Caenorhabditis elegans compared to avirulent bacteria. Co-feeding EHEC with Enterococcus faecalis Symbioflor(®) significantly increased the worms' lifespan. The transcriptome of EHEC grown in vitro with or without Symbioflor(®) was analyzed using RNA-seq. The analysis revealed downregulation of several virulence-associated genes in the presence of Symbioflor(®), including virulence key genes (e.g., LEE, flagellum, quorum-sensing). The downregulation of the LEE genes was corroborated by lux-transposon mutants. Upregulated genes included acid response genes, due to a decrease in pH exerted by Symbioflor(®). Further genes indicate cellular stress in EHEC (e.g. prophage/mobile elements involved in excision, cell lysis, and cell division inhibition). Thus, the observed protection of C. elegans during an EHEC infection by the probiotic Symbioflor(®) is suggested to be caused by triggering concomitant transcriptomic changes. To verify the biological relevance of this modulation, exemplary genes found to be influenced by Symbioflor(®) were knocked out (fliD, espB, Z3136, Z3917, and L7052). The lifespan of nematodes changed when using knock-outs as food source and the effect could be complemented in trans. In summary, Symbioflor(®) appears to be a protective probiotic in the nematode model.</dcterms:abstract>
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