Bacterial microcompartments for isethionate desulfonation in the taurine-degrading human-gut bacterium Bilophila wadsworthia

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Zusammenfassung

Background
Bilophila wadsworthia, a strictly anaerobic, sulfite-reducing bacterium and common member of the human gut microbiota, has been associated with diseases such as appendicitis and colitis. It is specialized on organosulfonate respiration for energy conservation, i.e., utilization of dietary and host-derived organosulfonates, such as taurine (2-aminoethansulfonate), as sulfite donors for sulfite respiration, producing hydrogen sulfide (H2S), an important intestinal metabolite that may have beneficial as well as detrimental effects on the colonic environment. Its taurine desulfonation pathway involves the glycyl radical enzyme (GRE) isethionate sulfite-lyase (IslAB), which cleaves isethionate (2-hydroxyethanesulfonate) into acetaldehyde and sulfite.

Results
We demonstrate that taurine metabolism in B. wadsworthia 3.1.6 involves bacterial microcompartments (BMCs). First, we confirmed taurine-inducible production of BMCs by proteomic, transcriptomic and ultra-thin sectioning and electron-microscopical analyses. Then, we isolated BMCs from taurine-grown cells by density-gradient ultracentrifugation and analyzed their composition by proteomics as well as by enzyme assays, which suggested that the GRE IslAB and acetaldehyde dehydrogenase are located inside of the BMCs. Finally, we are discussing the recycling of cofactors in the IslAB-BMCs and a potential shuttling of electrons across the BMC shell by a potential iron-sulfur (FeS) cluster-containing shell protein identified by sequence analysis.

Conclusions
We characterized a novel subclass of BMCs and broadened the spectrum of reactions known to take place enclosed in BMCs, which is of biotechnological interest. We also provided more details on the energy metabolism of the opportunistic pathobiont B. wadsworthia and on microbial H2S production in the human gut.

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ISO 690BURRICHTER, Anna Georga, Stefanie DÖRR, Paavo BERGMANN, Sebastian HAISS, Anja KELLER, Corentin FOURNIER, Paolo FRANCHINI, Erika ISONO, David SCHLEHECK, 2021. Bacterial microcompartments for isethionate desulfonation in the taurine-degrading human-gut bacterium Bilophila wadsworthia. In: BMC Microbiology. BioMed Central. 2021, 21, 340. eISSN 1471-2180. Available under: doi: 10.1186/s12866-021-02386-w
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@article{Burrichter2021-12-13Bacte-56311,
  year={2021},
  doi={10.1186/s12866-021-02386-w},
  title={Bacterial microcompartments for isethionate desulfonation in the taurine-degrading human-gut bacterium Bilophila wadsworthia},
  volume={21},
  journal={BMC Microbiology},
  author={Burrichter, Anna Georga and Dörr, Stefanie and Bergmann, Paavo and Haiß, Sebastian and Keller, Anja and Fournier, Corentin and Franchini, Paolo and Isono, Erika and Schleheck, David},
  note={Deutsche Forschungsgemeinschaft (DFG) (to DS; grant SCHL 1936/4) Article Number: 340}
}
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