Publikation: Low temperature or GroEL/ES overproduction permits growth of Escherichia coli cells lacking trigger factor and DnaK
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2004
Autor:innen
Vorderwülbecke, Sonja
Kramer, Günter
Merz, Frieder
Kurz, Thorben A.
Rauch, Thomas
Zachmann-Brand, Beate
Bukau, Bernd
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FEBS Letters. 2004, 559(1-3), pp. 181-187. ISSN 0014-5793. Available under: doi: 10.1016/S0014-5793(04)00052-3
Zusammenfassung
Escherichia coli trigger factor (TF) and DnaK cooperate in the folding of newly synthesized proteins. The combined deletion of the TF-encoding tig gene and the dnaK gene causes protein aggregation and synthetic lethality at 30°C. Here we show that the synthetic lethality of ΔtigΔdnaK52 cells is abrogated either by growth below 30°C or by overproduction of GroEL/GroES. At 23°C ΔtigΔdnaK52 cells were viable and showed only minor protein aggregation. Overproduction of GroEL/GroES, but not of other chaperones, restored growth of ΔtigΔdnaK52 cells at 30°C and suppressed protein aggregation including proteins ≥60kDa, which normally require TF and DnaK for folding. GroEL/GroES thus influences the folding of proteins previously identified as DnaK/TF substrates.
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Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
Chaperone, De novo folding, 2D gel electrophoresis, Nascent polypeptide, Protein aggregation
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VORDERWÜLBECKE, Sonja, Günter KRAMER, Frieder MERZ, Thorben A. KURZ, Thomas RAUCH, Beate ZACHMANN-BRAND, Bernd BUKAU, Elke DEUERLING, 2004. Low temperature or GroEL/ES overproduction permits growth of Escherichia coli cells lacking trigger factor and DnaK. In: FEBS Letters. 2004, 559(1-3), pp. 181-187. ISSN 0014-5793. Available under: doi: 10.1016/S0014-5793(04)00052-3BibTex
@article{Vorderwulbecke2004tempe-7841,
year={2004},
doi={10.1016/S0014-5793(04)00052-3},
title={Low temperature or GroEL/ES overproduction permits growth of Escherichia coli cells lacking trigger factor and DnaK},
number={1-3},
volume={559},
issn={0014-5793},
journal={FEBS Letters},
pages={181--187},
author={Vorderwülbecke, Sonja and Kramer, Günter and Merz, Frieder and Kurz, Thorben A. and Rauch, Thomas and Zachmann-Brand, Beate and Bukau, Bernd and Deuerling, Elke}
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<dcterms:abstract xml:lang="eng">Escherichia coli trigger factor (TF) and DnaK cooperate in the folding of newly synthesized proteins. The combined deletion of the TF-encoding tig gene and the dnaK gene causes protein aggregation and synthetic lethality at 30°C. Here we show that the synthetic lethality of ΔtigΔdnaK52 cells is abrogated either by growth below 30°C or by overproduction of GroEL/GroES. At 23°C ΔtigΔdnaK52 cells were viable and showed only minor protein aggregation. Overproduction of GroEL/GroES, but not of other chaperones, restored growth of ΔtigΔdnaK52 cells at 30°C and suppressed protein aggregation including proteins ≥60kDa, which normally require TF and DnaK for folding. GroEL/GroES thus influences the folding of proteins previously identified as DnaK/TF substrates.</dcterms:abstract>
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