Ubiquitin-fusion protein system : a powerful tool for ectopic protein expression in mammalian cells
| dc.contributor.author | Matentzoglu, Konstantin | |
| dc.contributor.author | Scheffner, Martin | |
| dc.date.accessioned | 2011-03-23T09:07:33Z | deu |
| dc.date.available | 2011-03-23T09:07:33Z | deu |
| dc.date.issued | 2009 | deu |
| dc.description.abstract | Clonal cell lines stably expressing a protein of interest are frequently used in the characterization of the potential physiological functions of mammalian proteins, but the generation of such cell lines is laborious and time-consuming. Here, we made use of the fact that fusion proteins consisting of ubiquitin linked to the N terminus of a protein of interest are efficiently processed by ubiquitin-specific proteases into their respective free proteins within eukaryotic cells, and designed an expression system that allows easy and highly efficient selection of mammalian cells ectopically expressing a protein of interest. | eng |
| dc.description.version | published | |
| dc.identifier.citation | Publ. in: BioTechniques 46 (2009), 1, pp. 21-28 | deu |
| dc.identifier.doi | 10.2144/000113023 | |
| dc.identifier.uri | http://kops.uni-konstanz.de/handle/123456789/1271 | |
| dc.language.iso | eng | deu |
| dc.legacy.dateIssued | 2009 | deu |
| dc.rights | terms-of-use | deu |
| dc.rights.uri | https://rightsstatements.org/page/InC/1.0/ | deu |
| dc.subject.ddc | 570 | deu |
| dc.subject.gnd | Ubiquitin | deu |
| dc.title | Ubiquitin-fusion protein system : a powerful tool for ectopic protein expression in mammalian cells | eng |
| dc.type | JOURNAL_ARTICLE | deu |
| dspace.entity.type | Publication | |
| kops.citation.bibtex | @article{Matentzoglu2009Ubiqu-1271,
year={2009},
doi={10.2144/000113023},
title={Ubiquitin-fusion protein system : a powerful tool for ectopic protein expression in mammalian cells},
number={1},
volume={46},
issn={0736-6205},
journal={BioTechniques},
pages={21--28},
author={Matentzoglu, Konstantin and Scheffner, Martin}
} | |
| kops.citation.iso690 | MATENTZOGLU, Konstantin, Martin SCHEFFNER, 2009. Ubiquitin-fusion protein system : a powerful tool for ectopic protein expression in mammalian cells. In: BioTechniques. 2009, 46(1), pp. 21-28. ISSN 0736-6205. eISSN 1940-9818. Available under: doi: 10.2144/000113023 | deu |
| kops.citation.iso690 | MATENTZOGLU, Konstantin, Martin SCHEFFNER, 2009. Ubiquitin-fusion protein system : a powerful tool for ectopic protein expression in mammalian cells. In: BioTechniques. 2009, 46(1), pp. 21-28. ISSN 0736-6205. eISSN 1940-9818. Available under: doi: 10.2144/000113023 | eng |
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