The diatom Phaeodactylum tricornutum adjusts nonphotochemical fluorescence quenching capacity in response to dynamic light via fine-tuned Lhcx and xanthophyll cycle pigment synthesis

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2017
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Gélin, Gautier
Rogato, Alessandra
Falciatore, Angela
Lavaud, Johann
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Diatoms contain a highly flexible capacity to dissipate excessively absorbed light by nonphotochemical fluorescence quenching (NPQ) based on the light-induced conversion of diadinoxanthin (Dd) into diatoxanthin (Dt) and the presence of Lhcx proteins. Their NPQ fine regulation on the molecular level upon a shift to dynamic light conditions is unknown.

We investigated the regulation of Dd + Dt amount, Lhcx gene and protein synthesis and NPQ capacity in the diatom Phaeodactylum tricornutum after a change from continuous low light to 3 d of sine (SL) or fluctuating (FL) light conditions. Four P. tricornutum strains with different NPQ capacities due to different expression of Lhcx1 were included.

All strains responded to dynamic light comparably, independently of initial NPQ capacity. During SL, NPQ capacity was strongly enhanced due to a gradual increase of Lhcx2 and Dd + Dt amount. During FL, cells enhanced their NPQ capacity on the first day due to increased Dd + Dt, Lhcx2 and Lhcx3; already by the second day light acclimation was accomplished. While quenching efficiency of Dt was strongly lowered during SL conditions, it remained high throughout the whole FL exposure.

Our results highlight a more balanced and cost-effective photoacclimation strategy of P. tricornutum under FL than under SL conditions.

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ISO 690LEPETIT, Bernard, Gautier GÉLIN, Mariana LEPETIT, Sabine STURM, Sascha VUGRINEC, Alessandra ROGATO, Peter G. KROTH, Angela FALCIATORE, Johann LAVAUD, 2017. The diatom Phaeodactylum tricornutum adjusts nonphotochemical fluorescence quenching capacity in response to dynamic light via fine-tuned Lhcx and xanthophyll cycle pigment synthesis. In: New Phytologist. 2017, 214(1), pp. 205-218. ISSN 0028-646X. eISSN 1469-8137. Available under: doi: 10.1111/nph.14337
BibTex
@article{Lepetit2017-04diato-36240,
  year={2017},
  doi={10.1111/nph.14337},
  title={The diatom Phaeodactylum tricornutum adjusts nonphotochemical fluorescence quenching capacity in response to dynamic light via fine-tuned Lhcx and xanthophyll cycle pigment synthesis},
  number={1},
  volume={214},
  issn={0028-646X},
  journal={New Phytologist},
  pages={205--218},
  author={Lepetit, Bernard and Gélin, Gautier and Lepetit, Mariana and Sturm, Sabine and Vugrinec, Sascha and Rogato, Alessandra and Kroth, Peter G. and Falciatore, Angela and Lavaud, Johann}
}
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