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The ubiquitin-like proteins SMT3 and SUMO-1 are conjugated by the UBC9 E2 enzyme

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1998

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Schwarz, Sylvia E.
Matuschewski, Kai
Liakopoulos, Dimitris
Jentsch, Stefan

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https://doi.org/10.1073/pnas.95.2.560
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Proceedings of the National Academy of Sciences. 1998, 95(2), pp. 560-564. ISSN 0027-8424. eISSN 1091-6490. Available under: doi: 10.1073/pnas.95.2.560

Zusammenfassung

The ubiquitin-like protein SMT3 from Saccharomyces cerevisiae and SUMO-1, its mammalian homolog, can be covalently attached to other proteins posttranslationally. Conjugation of ubiquitin requires the activities of ubiquitin- activating (E1) and -conjugating (E2) enzymes and proceeds via thioester-linked enzyme-ubiquitin intermediates. Herein we show that UBC9, one of the 13 different E2 enzymes from yeast, is required for SMT3 conjugation in vivo. Moreover, recombinant yeast and mammalian UBC9 enzymes were found to form thioester complexes with SMT3 and SUMO-1, respectively. This suggests that UBC9 functions as an E2 in a SMT3ySUMO-1 conjugation pathway analogous to ubiquitinconjugating enzymes. The role of yeast UBC9 in cell cycle progression may thus be mediated through its SMT3 conjugation activity.

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570 Biowissenschaften, Biologie

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ISO 690SCHWARZ, Sylvia E., Kai MATUSCHEWSKI, Dimitris LIAKOPOULOS, Martin SCHEFFNER, Stefan JENTSCH, 1998. The ubiquitin-like proteins SMT3 and SUMO-1 are conjugated by the UBC9 E2 enzyme. In: Proceedings of the National Academy of Sciences. 1998, 95(2), pp. 560-564. ISSN 0027-8424. eISSN 1091-6490. Available under: doi: 10.1073/pnas.95.2.560
BibTex
@article{Schwarz1998ubiqu-42782,
  year={1998},
  doi={10.1073/pnas.95.2.560},
  title={The ubiquitin-like proteins SMT3 and SUMO-1 are conjugated by the UBC9 E2 enzyme},
  number={2},
  volume={95},
  issn={0027-8424},
  journal={Proceedings of the National Academy of Sciences},
  pages={560--564},
  author={Schwarz, Sylvia E. and Matuschewski, Kai and Liakopoulos, Dimitris and Scheffner, Martin and Jentsch, Stefan}
}
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