Isolation of Plastid Fractions from the Diatoms Thalassiosira pseudonana and Phaeodactylum tricornutum

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2018
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MARÉCHAL, Eric, ed.. Plastids : methods and protocols. New York: Humana Press, 2018, pp. 189-203. ISBN 978-1-4939-8653-8. Available under: doi: 10.1007/978-1-4939-8654-5_13
Zusammenfassung

The so-called "complex" plastids from diatoms possessing four envelope membranes are a typical feature of algae that arose from secondary endosymbiosis. Studying isolated plastids from these algae may allow answering a number of fundamental questions regarding diatom photosynthesis and plastid functionality. Due to their complex architecture and their integration into the cellular endoplasmic reticulum (ER) system, their isolation though is still challenging. In this work, we report a reliable isolation technique that is applicable for the two model diatoms Thalassiosira pseudonana and Phaeodactylum tricornutum. The resulting plastid-enriched fractions are of homogenous quality, almost free from cellular contaminants, and feature structurally intact thylakoids that are capable to perform oxygenic photosynthesis, though in most cases they seem to lack most of the stromal components as well as plastid envelopes.

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570 Biowissenschaften, Biologie
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Diatom organelles, Thylakoids, Complex plastid fractionation, Chloroplast
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ISO 690SCHOBER, Alexander, Serena FLORI, Giovanni FINAZZI, Peter G. KROTH, Carolina RÍO BÁRTULOS, 2018. Isolation of Plastid Fractions from the Diatoms Thalassiosira pseudonana and Phaeodactylum tricornutum. In: MARÉCHAL, Eric, ed.. Plastids : methods and protocols. New York: Humana Press, 2018, pp. 189-203. ISBN 978-1-4939-8653-8. Available under: doi: 10.1007/978-1-4939-8654-5_13
BibTex
@incollection{Schober2018Isola-42938,
  year={2018},
  doi={10.1007/978-1-4939-8654-5_13},
  title={Isolation of Plastid Fractions from the Diatoms Thalassiosira pseudonana and Phaeodactylum tricornutum},
  isbn={978-1-4939-8653-8},
  publisher={Humana Press},
  address={New York},
  booktitle={Plastids : methods and protocols},
  pages={189--203},
  editor={Maréchal, Eric},
  author={Schober, Alexander and Flori, Serena and Finazzi, Giovanni and Kroth, Peter G. and Río Bártulos, Carolina}
}
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