Publikation: Quantitative Analysis of Gene Expression Relative to 18S rRNA in Carcinoma Samples Using the LightCycler® Instrument and a SYBR GreenI-based Assay: Determining FAT10 mRNA Levels in Hepatocellular Carcinoma
Dateien
Datum
Autor:innen
Herausgeber:innen
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
URI (zitierfähiger Link)
Internationale Patentnummer
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Sammlungen
Core Facility der Universität Konstanz
Titel in einer weiteren Sprache
Publikationstyp
Publikationsstatus
Erschienen in
Zusammenfassung
Due to the fact that mutations and up- or downregulation of genes can lead to the development of cancer, quantitative comparison of relative gene expression in healthy and cancerous tissue can gain valuable insights into tumorigenesis. While the semi-quantitative DNA microarrays are being used to identify differentially expressed genes on a genomic scale, real-time RT-PCR provides a powerful tool for quantitative measurement of gene expression. Presently, it is the most sensitive method available. Here we describe in detail a SYBR GreenI-based assay using the LightCycler® instrument to measure the levels of mRNA for the ubiquitin-like protein FAT10 relative to 18S rRNA in human hepatocellular carcinoma tissue. This method can be easily adapted to any tissue (human or mouse, rat, etc.) and any gene.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
Schlagwörter
Konferenz
Rezension
Zitieren
ISO 690
LUKASIAK, Sebastian, Kai BREUHAHN, Claudia SCHILLER, Gunter SCHMIDTKE, Marcus GRÖTTRUP, 2008. Quantitative Analysis of Gene Expression Relative to 18S rRNA in Carcinoma Samples Using the LightCycler® Instrument and a SYBR GreenI-based Assay: Determining FAT10 mRNA Levels in Hepatocellular Carcinoma. In: MARX, Andreas, ed., Oliver SEITZ, ed.. Molecular beacons : signalling nucleic acid probes, methods, and protocols. Totowa, NJ: Humana Press, 2008, pp. 59-72. Methods in molecular biology. 429. ISBN 978-1-58829-700-6BibTex
@incollection{Lukasiak2008Quant-1270, year={2008}, title={Quantitative Analysis of Gene Expression Relative to 18S rRNA in Carcinoma Samples Using the LightCycler® Instrument and a SYBR GreenI-based Assay: Determining FAT10 mRNA Levels in Hepatocellular Carcinoma}, number={429}, isbn={978-1-58829-700-6}, publisher={Humana Press}, address={Totowa, NJ}, series={Methods in molecular biology}, booktitle={Molecular beacons : signalling nucleic acid probes, methods, and protocols}, pages={59--72}, editor={Marx, Andreas and Seitz, Oliver}, author={Lukasiak, Sebastian and Breuhahn, Kai and Schiller, Claudia and Schmidtke, Gunter and Gröttrup, Marcus} }
RDF
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/1270"> <dcterms:bibliographicCitation>Publ. in: Molecular beacons : signalling nucleic acid probes, methods, and protocols / ed. by Andreas Marx and Oliver Seitz. - Totowa, NJ : Humana Press, 2008, pp. 59-72. - (Methods in molecular biology ; 429)</dcterms:bibliographicCitation> <dc:contributor>Schmidtke, Gunter</dc:contributor> <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/1270"/> <dc:language>eng</dc:language> <dc:creator>Lukasiak, Sebastian</dc:creator> <dc:creator>Schmidtke, Gunter</dc:creator> <dc:creator>Gröttrup, Marcus</dc:creator> <dc:creator>Breuhahn, Kai</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-23T09:07:33Z</dcterms:available> <dcterms:title>Quantitative Analysis of Gene Expression Relative to 18S rRNA in Carcinoma Samples Using the LightCycler® Instrument and a SYBR GreenI-based Assay: Determining FAT10 mRNA Levels in Hepatocellular Carcinoma</dcterms:title> <dc:creator>Schiller, Claudia</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-23T09:07:33Z</dc:date> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dc:contributor>Gröttrup, Marcus</dc:contributor> <dc:rights>terms-of-use</dc:rights> <dcterms:issued>2008</dcterms:issued> <dc:contributor>Breuhahn, Kai</dc:contributor> <dc:contributor>Lukasiak, Sebastian</dc:contributor> <dc:contributor>Schiller, Claudia</dc:contributor> <foaf:homepage rdf:resource="http://localhost:8080/"/> <dcterms:abstract xml:lang="eng">Due to the fact that mutations and up- or downregulation of genes can lead to the development of cancer, quantitative comparison of relative gene expression in healthy and cancerous tissue can gain valuable insights into tumorigenesis. While the semi-quantitative DNA microarrays are being used to identify differentially expressed genes on a genomic scale, real-time RT-PCR provides a powerful tool for quantitative measurement of gene expression. Presently, it is the most sensitive method available. Here we describe in detail a SYBR GreenI-based assay using the LightCycler® instrument to measure the levels of mRNA for the ubiquitin-like protein FAT10 relative to 18S rRNA in human hepatocellular carcinoma tissue. This method can be easily adapted to any tissue (human or mouse, rat, etc.) and any gene.</dcterms:abstract> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> </rdf:Description> </rdf:RDF>