@incollection{Lukasiak2008Quant-1270,
  year={2008},
  title={Quantitative Analysis of Gene Expression Relative to 18S rRNA in Carcinoma Samples Using the LightCycler® Instrument and a SYBR GreenI-based Assay: Determining FAT10 mRNA Levels in Hepatocellular Carcinoma},
  number={429},
  isbn={978-1-58829-700-6},
  publisher={Humana Press},
  address={Totowa, NJ},
  series={Methods in molecular biology},
  booktitle={Molecular beacons : signalling nucleic acid probes, methods, and protocols},
  pages={59--72},
  editor={Marx, Andreas and Seitz, Oliver},
  author={Lukasiak, Sebastian and Breuhahn, Kai and Schiller, Claudia and Schmidtke, Gunter and Gröttrup, Marcus}
}

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    <dcterms:bibliographicCitation>Publ. in: Molecular beacons : signalling nucleic acid probes, methods, and protocols / ed. by Andreas Marx and Oliver Seitz. - Totowa, NJ : Humana Press, 2008, pp. 59-72. - (Methods in molecular biology ; 429)</dcterms:bibliographicCitation>
    <dc:contributor>Schmidtke, Gunter</dc:contributor>
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    <dc:language>eng</dc:language>
    <dc:creator>Lukasiak, Sebastian</dc:creator>
    <dc:creator>Schmidtke, Gunter</dc:creator>
    <dc:creator>Gröttrup, Marcus</dc:creator>
    <dc:creator>Breuhahn, Kai</dc:creator>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-23T09:07:33Z</dcterms:available>
    <dcterms:title>Quantitative Analysis of Gene Expression Relative to 18S rRNA in Carcinoma Samples Using the LightCycler® Instrument and a SYBR GreenI-based Assay: Determining FAT10 mRNA Levels in Hepatocellular Carcinoma</dcterms:title>
    <dc:creator>Schiller, Claudia</dc:creator>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-23T09:07:33Z</dc:date>
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    <dc:contributor>Gröttrup, Marcus</dc:contributor>
    <dc:rights>terms-of-use</dc:rights>
    <dcterms:issued>2008</dcterms:issued>
    <dc:contributor>Breuhahn, Kai</dc:contributor>
    <dc:contributor>Lukasiak, Sebastian</dc:contributor>
    <dc:contributor>Schiller, Claudia</dc:contributor>
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    <dcterms:abstract xml:lang="eng">Due to the fact that mutations and up- or downregulation of genes can lead to the development of cancer, quantitative comparison of relative gene expression in healthy and cancerous tissue can gain valuable insights into tumorigenesis. While the semi-quantitative DNA microarrays are being used to identify differentially expressed genes on a genomic scale, real-time RT-PCR provides a powerful tool for quantitative measurement of gene expression. Presently, it is the most sensitive method available. Here we describe in detail a SYBR GreenI-based assay using the LightCycler® instrument to measure the levels of mRNA for the ubiquitin-like protein FAT10 relative to 18S rRNA in human hepatocellular carcinoma tissue. This method can be easily adapted to any tissue (human or mouse, rat, etc.) and any gene.</dcterms:abstract>
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