Publikation:

Quantitative Analysis of Gene Expression Relative to 18S rRNA in Carcinoma Samples Using the LightCycler® Instrument and a SYBR GreenI-based Assay: Determining FAT10 mRNA Levels in Hepatocellular Carcinoma

Vorschaubild

Dateien

Zu diesem Dokument gibt es keine Dateien.

Datum

2008

Autor:innen

Lukasiak, Sebastian
Breuhahn, Kai
Schiller, Claudia

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

URI (zitierfähiger Link)
http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-119832
DOI (zitierfähiger Link)
ArXiv-ID

Internationale Patentnummer

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung

Sammlungen

Biologie: Publikationen
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Beitrag zu einem Sammelband
Publikationsstatus
Published

Erschienen in

MARX, Andreas, ed., Oliver SEITZ, ed.. Molecular beacons : signalling nucleic acid probes, methods, and protocols. Totowa, NJ: Humana Press, 2008, pp. 59-72. Methods in molecular biology. 429. ISBN 978-1-58829-700-6

Zusammenfassung

Due to the fact that mutations and up- or downregulation of genes can lead to the development of cancer, quantitative comparison of relative gene expression in healthy and cancerous tissue can gain valuable insights into tumorigenesis. While the semi-quantitative DNA microarrays are being used to identify differentially expressed genes on a genomic scale, real-time RT-PCR provides a powerful tool for quantitative measurement of gene expression. Presently, it is the most sensitive method available. Here we describe in detail a SYBR GreenI-based assay using the LightCycler® instrument to measure the levels of mRNA for the ubiquitin-like protein FAT10 relative to 18S rRNA in human hepatocellular carcinoma tissue. This method can be easily adapted to any tissue (human or mouse, rat, etc.) and any gene.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Relative gene expression, quantitative RT-PCR, SYBR GreenI, co-application reverse transcription (Co-RT), normalization to 18S rRNA

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690LUKASIAK, Sebastian, Kai BREUHAHN, Claudia SCHILLER, Gunter SCHMIDTKE, Marcus GRÖTTRUP, 2008. Quantitative Analysis of Gene Expression Relative to 18S rRNA in Carcinoma Samples Using the LightCycler® Instrument and a SYBR GreenI-based Assay: Determining FAT10 mRNA Levels in Hepatocellular Carcinoma. In: MARX, Andreas, ed., Oliver SEITZ, ed.. Molecular beacons : signalling nucleic acid probes, methods, and protocols. Totowa, NJ: Humana Press, 2008, pp. 59-72. Methods in molecular biology. 429. ISBN 978-1-58829-700-6
BibTex
@incollection{Lukasiak2008Quant-1270,
  year={2008},
  title={Quantitative Analysis of Gene Expression Relative to 18S rRNA in Carcinoma Samples Using the LightCycler® Instrument and a SYBR GreenI-based Assay: Determining FAT10 mRNA Levels in Hepatocellular Carcinoma},
  number={429},
  isbn={978-1-58829-700-6},
  publisher={Humana Press},
  address={Totowa, NJ},
  series={Methods in molecular biology},
  booktitle={Molecular beacons : signalling nucleic acid probes, methods, and protocols},
  pages={59--72},
  editor={Marx, Andreas and Seitz, Oliver},
  author={Lukasiak, Sebastian and Breuhahn, Kai and Schiller, Claudia and Schmidtke, Gunter and Gröttrup, Marcus}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/1270">
    <dcterms:bibliographicCitation>Publ. in: Molecular beacons : signalling nucleic acid probes, methods, and protocols / ed. by Andreas Marx and Oliver Seitz. - Totowa, NJ : Humana Press, 2008, pp. 59-72. - (Methods in molecular biology ; 429)</dcterms:bibliographicCitation>
    <dc:contributor>Schmidtke, Gunter</dc:contributor>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/1270"/>
    <dc:language>eng</dc:language>
    <dc:creator>Lukasiak, Sebastian</dc:creator>
    <dc:creator>Schmidtke, Gunter</dc:creator>
    <dc:creator>Gröttrup, Marcus</dc:creator>
    <dc:creator>Breuhahn, Kai</dc:creator>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-23T09:07:33Z</dcterms:available>
    <dcterms:title>Quantitative Analysis of Gene Expression Relative to 18S rRNA in Carcinoma Samples Using the LightCycler® Instrument and a SYBR GreenI-based Assay: Determining FAT10 mRNA Levels in Hepatocellular Carcinoma</dcterms:title>
    <dc:creator>Schiller, Claudia</dc:creator>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-23T09:07:33Z</dc:date>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Gröttrup, Marcus</dc:contributor>
    <dc:rights>terms-of-use</dc:rights>
    <dcterms:issued>2008</dcterms:issued>
    <dc:contributor>Breuhahn, Kai</dc:contributor>
    <dc:contributor>Lukasiak, Sebastian</dc:contributor>
    <dc:contributor>Schiller, Claudia</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:abstract xml:lang="eng">Due to the fact that mutations and up- or downregulation of genes can lead to the development of cancer, quantitative comparison of relative gene expression in healthy and cancerous tissue can gain valuable insights into tumorigenesis. While the semi-quantitative DNA microarrays are being used to identify differentially expressed genes on a genomic scale, real-time RT-PCR provides a powerful tool for quantitative measurement of gene expression. Presently, it is the most sensitive method available. Here we describe in detail a SYBR GreenI-based assay using the LightCycler® instrument to measure the levels of mRNA for the ubiquitin-like protein FAT10 relative to 18S rRNA in human hepatocellular carcinoma tissue. This method can be easily adapted to any tissue (human or mouse, rat, etc.) and any gene.</dcterms:abstract>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Diese Publikation teilen