Publikation:

Low-resolution structure of recombinant human granulocyte-macrophage colony stimulating factor

Lade...
Vorschaubild

Dateien

diederichs_208694.pdf
diederichs_208694.pdfGröße: 469.89 KBDownloads: 395

Datum

1991

Autor:innen

Jacques, Steven
Boone, Tom
Karplus, P. Andrew

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

ArXiv-ID

Internationale Patentnummer

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung
Open Access Green
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

Journal of Molecular Biology. 1991, 221(1), pp. 55-60. ISSN 0022-2836. eISSN 1089-8638. Available under: doi: 10.1016/0022-2836(91)80203-7

Zusammenfassung

A recombinant form of human granulocyte-macrophage colony stimulating factor (GM-CSF) which contains no carbohydrate has been crystallized. Multiple isomorphous replacement analysis using five heavy-atom derivatives has yielded an image of the structure at 6Åresolution that showed two molecules per asymmetric unit and allowed determination of the non-crystallographic symmetry transformation. The 6Åresolution result shows that the core of GM-CSF consists of four helices. The angles at which the helices pack together distinguishes this structure from known antiparallel four-helix bundle proteins. Consideration of the amino acid sequence properties and previous structural characterizations of GM-CSF leads to an assignment of the probable protein segments that form the helices.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Granulocyte-macrophage colony stimulating factor, lymphokine, crystallization, X-ray diffraction, protein structure

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690DIEDERICHS, Kay, Steven JACQUES, Tom BOONE, P. Andrew KARPLUS, 1991. Low-resolution structure of recombinant human granulocyte-macrophage colony stimulating factor. In: Journal of Molecular Biology. 1991, 221(1), pp. 55-60. ISSN 0022-2836. eISSN 1089-8638. Available under: doi: 10.1016/0022-2836(91)80203-7
BibTex
@article{Diederichs1991Lowre-20869,
  year={1991},
  doi={10.1016/0022-2836(91)80203-7},
  title={Low-resolution structure of recombinant human granulocyte-macrophage colony stimulating factor},
  number={1},
  volume={221},
  issn={0022-2836},
  journal={Journal of Molecular Biology},
  pages={55--60},
  author={Diederichs, Kay and Jacques, Steven and Boone, Tom and Karplus, P. Andrew}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/20869">
    <dc:creator>Boone, Tom</dc:creator>
    <dc:contributor>Karplus, P. Andrew</dc:contributor>
    <dc:creator>Karplus, P. Andrew</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Diederichs, Kay</dc:creator>
    <dc:contributor>Diederichs, Kay</dc:contributor>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-11-16T06:49:22Z</dcterms:available>
    <dc:language>eng</dc:language>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-11-16T06:49:22Z</dc:date>
    <dcterms:issued>1991</dcterms:issued>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:bibliographicCitation>Journal of Molecular Biology ; 221 (1991), 1. - S. 55-60</dcterms:bibliographicCitation>
    <dc:rights>terms-of-use</dc:rights>
    <dc:creator>Jacques, Steven</dc:creator>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:contributor>Jacques, Steven</dc:contributor>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/20869/2/diederichs_208694.pdf"/>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dc:contributor>Boone, Tom</dc:contributor>
    <dcterms:abstract xml:lang="eng">A recombinant form of human granulocyte-macrophage colony stimulating factor (GM-CSF) which contains no carbohydrate has been crystallized. Multiple isomorphous replacement analysis using five heavy-atom derivatives has yielded an image of the structure at 6Åresolution that showed two molecules per asymmetric unit and allowed determination of the non-crystallographic symmetry transformation. The 6Åresolution result shows that the core of GM-CSF consists of four helices. The angles at which the helices pack together distinguishes this structure from known antiparallel four-helix bundle proteins. Consideration of the amino acid sequence properties and previous structural characterizations of GM-CSF leads to an assignment of the probable protein segments that form the helices.</dcterms:abstract>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/20869/2/diederichs_208694.pdf"/>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/20869"/>
    <dcterms:title>Low-resolution structure of recombinant human granulocyte-macrophage colony stimulating factor</dcterms:title>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
Begutachtet
Diese Publikation teilen