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Fast and long term lipid droplet tracking with CARS microscopy

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2011

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http://nbn-resolving.de/urn:nbn:de:bsz:352-193020
DOI (zitierfähiger Link)
https://doi.org/10.1002/jbio.201000120
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Chemie: Publikationen
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Published

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Journal of Biophotonics. 2011, 4(6), pp. 435-441. ISSN 1864-063X. eISSN 1864-0648. Available under: doi: 10.1002/jbio.201000120

Zusammenfassung

Photobleaching of organic fluorophores commonly used in fluorescence microscopy puts a limit to the number of images which can be acquired. Label-free imaging techniques therefore offer advantages both for rapid image acquisition and for long–term observations. CARS microscopy is a label-free imaging technique offering molecule specific contrast. Here we demonstrate that CARS microscopy allows video-rate tracking of intracellular transport of lipid droplets, but also continuous long-term observation of cells over several hours.

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Fachgebiet (DDC)
540 Chemie

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label-free imaging, particle tracking, CARS microscopy, lipid droplets

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ISO 690JÜNGST, Christian, Martin WINTERHALDER, Andreas ZUMBUSCH, 2011. Fast and long term lipid droplet tracking with CARS microscopy. In: Journal of Biophotonics. 2011, 4(6), pp. 435-441. ISSN 1864-063X. eISSN 1864-0648. Available under: doi: 10.1002/jbio.201000120
BibTex
@article{Jungst2011-06lipid-19302,
  year={2011},
  doi={10.1002/jbio.201000120},
  title={Fast and long term lipid droplet tracking with CARS microscopy},
  number={6},
  volume={4},
  issn={1864-063X},
  journal={Journal of Biophotonics},
  pages={435--441},
  author={Jüngst, Christian and Winterhalder, Martin and Zumbusch, Andreas}
}
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