Laser-Induced Magnetic Dipole Spectroscopy

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Pulse electron paramagnetic resonance measurements of nanometer scale distance distributions have proven highly effective in structural studies. They exploit the magnetic dipole-dipole coupling between spin labels site-specifically attached to macromolecules. The most commonly applied technique is double electron-electron resonance (DEER, also called pulsed electron double resonance (PELDOR)). Here we present the new technique of laser-induced magnetic dipole (LaserIMD) spectroscopy based on optical switching of the dipole-dipole coupling. In a proof of concept experiment on a model peptide, we find, already at a low quantum yield of triplet excitation, the same sensitivity for measuring the distance between a porphyrin and a nitroxide label as in a DEER measurement between two nitroxide labels. On the heme protein cytochrome C, we demonstrate that LaserIMD allows for distance measurements between a heme prosthetic group and a nitroxide label, although the heme triplet state is not directly observable by an electron spin echo.

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ISO 690HINTZE, Christian, Dennis BÜCKER, Silvia DOMINGO KÖHLER, Gunnar JESCHKE, Malte DRESCHER, 2016. Laser-Induced Magnetic Dipole Spectroscopy. In: The Journal of Physical Chemistry Letters. 2016, 7(12), pp. 2204-2209. eISSN 1948-7185. Available under: doi: 10.1021/acs.jpclett.6b00765
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@article{Hintze2016-06-16Laser-35203,
  year={2016},
  doi={10.1021/acs.jpclett.6b00765},
  title={Laser-Induced Magnetic Dipole Spectroscopy},
  number={12},
  volume={7},
  journal={The Journal of Physical Chemistry Letters},
  pages={2204--2209},
  author={Hintze, Christian and Bücker, Dennis and Domingo Köhler, Silvia and Jeschke, Gunnar and Drescher, Malte}
}
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    <dcterms:abstract xml:lang="eng">Pulse electron paramagnetic resonance measurements of nanometer scale distance distributions have proven highly effective in structural studies. They exploit the magnetic dipole-dipole coupling between spin labels site-specifically attached to macromolecules. The most commonly applied technique is double electron-electron resonance (DEER, also called pulsed electron double resonance (PELDOR)). Here we present the new technique of laser-induced magnetic dipole (LaserIMD) spectroscopy based on optical switching of the dipole-dipole coupling. In a proof of concept experiment on a model peptide, we find, already at a low quantum yield of triplet excitation, the same sensitivity for measuring the distance between a porphyrin and a nitroxide label as in a DEER measurement between two nitroxide labels. On the heme protein cytochrome C, we demonstrate that LaserIMD allows for distance measurements between a heme prosthetic group and a nitroxide label, although the heme triplet state is not directly observable by an electron spin echo.</dcterms:abstract>
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