Publikation: Laser-Induced Magnetic Dipole Spectroscopy
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Pulse electron paramagnetic resonance measurements of nanometer scale distance distributions have proven highly effective in structural studies. They exploit the magnetic dipole-dipole coupling between spin labels site-specifically attached to macromolecules. The most commonly applied technique is double electron-electron resonance (DEER, also called pulsed electron double resonance (PELDOR)). Here we present the new technique of laser-induced magnetic dipole (LaserIMD) spectroscopy based on optical switching of the dipole-dipole coupling. In a proof of concept experiment on a model peptide, we find, already at a low quantum yield of triplet excitation, the same sensitivity for measuring the distance between a porphyrin and a nitroxide label as in a DEER measurement between two nitroxide labels. On the heme protein cytochrome C, we demonstrate that LaserIMD allows for distance measurements between a heme prosthetic group and a nitroxide label, although the heme triplet state is not directly observable by an electron spin echo.
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HINTZE, Christian, Dennis BÜCKER, Silvia DOMINGO KÖHLER, Gunnar JESCHKE, Malte DRESCHER, 2016. Laser-Induced Magnetic Dipole Spectroscopy. In: The Journal of Physical Chemistry Letters. 2016, 7(12), pp. 2204-2209. eISSN 1948-7185. Available under: doi: 10.1021/acs.jpclett.6b00765BibTex
@article{Hintze2016-06-16Laser-35203, year={2016}, doi={10.1021/acs.jpclett.6b00765}, title={Laser-Induced Magnetic Dipole Spectroscopy}, number={12}, volume={7}, journal={The Journal of Physical Chemistry Letters}, pages={2204--2209}, author={Hintze, Christian and Bücker, Dennis and Domingo Köhler, Silvia and Jeschke, Gunnar and Drescher, Malte} }
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