Transcriptional activation of Igl, the gene for indole formation in Zea mays: a structure–activity study with elicitor-active N-acyl glutamines from insects

Frey, Monika; Spiteller, Dieter; Boland, Wilhelm; Gierl, Alfons

Transcriptional activation of Igl, the gene for indole formation in Zea mays: a structure–activity study with elicitor-active N-acyl glutamines from insects

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Date

2004

Authors

Frey, Monika

Spiteller, Dieter

Boland, Wilhelm

Gierl, Alfons

Publication type

Journal article

Published in

Phytochemistry ; 65 (2004), 8. - pp. 1047-1055. - ISSN 0031-9422

Abstract

The indole-3-glycerol phosphate lyase Igl is the structural gene of volatile indole biosynthesis in the tritrophic interaction in maize. The gene is activated on transcriptional level with the same kinetics and to the same level by the fatty acid–amino acid conjugates (FAC's) volicitin (17S)-(N-(17-hydroxylinolenoyl)-l-glutamine) and N-linolenoyl-l-glutamine. Both conjugates are present in the regurgitates of herbivorous caterpillars. Modifications of the fatty acid moiety of the FACs greatly reduces the elicitation of Igl and only the l-stereo-isomer of the FACs shows biological activity in the system. Volicitin treatment leads to a fast increase of AOS and AOC transcription levels and methyl jasmonate application induces Igl transcription. Hence, the induction of jasmonate biosynthesis appears to be an integral part of the elicitor mediated increase of Igl gene transcription.

The structural requirements of fatty acid–amino acid conjugates for induction of Igl transcription were investigated. A link to jasmonate signaling is described.

Subject (DDC)

570 Biosciences, Biology

Keywords

N-Acyl glutamines,Volicitin,Indole-3-glycerol phosphate lyase,Allene oxide synthase,Allene oxide cyclase,Jasmonate,Induced defense ACC,1-aminocyclopropane-1-carboxylic acid,AcSA,acetylsalicylic acid,AOC,allene oxide cyclase,AOS,allene oxide synthase,EST,expressed sequence tag,FAC,fatty acid–amino acid conjugate,GAP C,glycerol phosphate dehydrogenase,cytosolic form,Gln,glutamine,Glu,glutamate,JA,jasmonate,MeJa,methyl jasmonate,PCR,polymerase chain reaction

Cite This

ISO 690

FREY, Monika, Dieter SPITELLER, Wilhelm BOLAND, Alfons GIERL, 2004. Transcriptional activation of Igl, the gene for indole formation in Zea mays: a structure–activity study with elicitor-active N-acyl glutamines from insects. In: Phytochemistry. 65(8), pp. 1047-1055. ISSN 0031-9422. Available under: doi: 10.1016/j.phytochem.2003.11.021

BibTex

@article{Frey2004-04Trans-15517,
  year={2004},
  doi={10.1016/j.phytochem.2003.11.021},
  title={Transcriptional activation of Igl, the gene for indole formation in Zea mays: a structure–activity study with elicitor-active N-acyl glutamines from insects},
  number={8},
  volume={65},
  issn={0031-9422},
  journal={Phytochemistry},
  pages={1047--1055},
  author={Frey, Monika and Spiteller, Dieter and Boland, Wilhelm and Gierl, Alfons}
}

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