FAST TRACK : Identification of Reggie-1 and Reggie-2 as Plasmamembrane-Associated Proteins Which Cocluster with Activated GPI-Anchored Cell Adhesion Molecules in Non-Caveolar Micropatches in Neurons

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1998
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Lang, Dirk M.
Lommel, Silvia
Jung, Marion
Ankerhold, Richard
Petrausch, Barbara
Laessing, Ute
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Journal of Neurobiology. 1998, 37(4), pp. 502-523. ISSN 0022-3034. eISSN 1097-4695. Available under: doi: 10.1002/(SICI)1097-4695(199812)37:4<502::AID-NEU2>3.0.CO;2-S
Zusammenfassung

Neurons are believed to possess plasmalemmal microdomains and proteins analogous to the caveolae and caveolin of nonneuronal cells. Caveolae are plasmalemmal invaginations where activated glycosylphosphatidylinositol (GPI)-anchored proteins preferentially assemble and where transmembrane signaling may occur. Molecular cloning of rat reggie-1 and -2 (80% identical to goldfish reggie proteins) shows that reggie-2 is practically identical to mouse flotillin-1. Flotillin-1 and epidermal surface antigen (ESA) (flotillin-2) are suggested to represent possible membrane proteins in caveolae. Rat reggie-1 is 99% homologous to ESA in overlapping sequences but has a 49-amino-acid N-terminus not present in ESA. Antibodies (ABs) which recognize reggie-1 or -2 reveal that both proteins cluster at the plasmamembrane and occur in micropatches in neurons
[dorsal root ganglia (DRGs), retinal ganglion, and PC-12 cells] and in nonneuronal cells. In neurons, reggie micropatches occur along the axon and in lamellipodia and filopodia of growth cones, but they do not occur in caveolae. By quantitative electronmicroscopic analysis we demonstrate the absence of caveolae in (anti-caveolin negative) neurons and show anti-reggie-1 immunogoldlabeled clusters at the plasmamembrane of DRGs. When ABs against the GPI-anchored cell adhesion molecules (CAMs) F3 and Thy-1 are applied to live DRGs, the GPI-linked CAMs sequester into micropatches. Double immunofluorescence shows a colocalization of the CAMs with micropatches of anti-reggie antibodies. Thus, reggie-1 and reggie-2 identify sites where activated GPIlinked CAMs preferentially accumulate and which may represent noncaveolar micropatches (domains).

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570 Biowissenschaften, Biologie
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microdomains, neurons, quantitative EM analysis, GPI-anchored CAMs, coclustering, reggie-1 and -2
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ISO 690LANG, Dirk M., Silvia LOMMEL, Marion JUNG, Richard ANKERHOLD, Barbara PETRAUSCH, Ute LAESSING, Marianne F. WIECHERS, Helmut PLATTNER, Claudia STÜRMER, 1998. FAST TRACK : Identification of Reggie-1 and Reggie-2 as Plasmamembrane-Associated Proteins Which Cocluster with Activated GPI-Anchored Cell Adhesion Molecules in Non-Caveolar Micropatches in Neurons. In: Journal of Neurobiology. 1998, 37(4), pp. 502-523. ISSN 0022-3034. eISSN 1097-4695. Available under: doi: 10.1002/(SICI)1097-4695(199812)37:4<502::AID-NEU2>3.0.CO;2-S
BibTex
@article{Lang1998TRACK-6692,
  year={1998},
  doi={10.1002/(SICI)1097-4695(199812)37:4<502::AID-NEU2>3.0.CO;2-S},
  title={FAST TRACK : Identification of Reggie-1 and Reggie-2 as Plasmamembrane-Associated Proteins Which Cocluster with Activated GPI-Anchored Cell Adhesion Molecules in Non-Caveolar Micropatches in Neurons},
  number={4},
  volume={37},
  issn={0022-3034},
  journal={Journal of Neurobiology},
  pages={502--523},
  author={Lang, Dirk M. and Lommel, Silvia and Jung, Marion and Ankerhold, Richard and Petrausch, Barbara and Laessing, Ute and Wiechers, Marianne F. and Plattner, Helmut and Stürmer, Claudia}
}
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