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Engineering Aptazyme Switches for Conditional Gene Expression in Mammalian Cells Utilizing an In Vivo Screening Approach

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2015

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https://doi.org/10.1007/978-1-4939-2730-2_11
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PONCHON, Luc, ed.. RNA Scaffolds : Methods and Protocols. New York: Springer, 2015, pp. 127-140. Methods in Molecular Biology. 1316. ISBN 978-1-4939-2729-6. Available under: doi: 10.1007/978-1-4939-2730-2_11

Zusammenfassung

Artificial RNA switches are an emerging class of genetic controllers suitable for synthetic biology applications. Aptazymes are fusions composed of an aptamer domain and a self-cleaving ribozyme. The utilization of aptazymes for conditional gene expression displays several advantages over employing conventional transcription factor-based techniques as aptazymes require minimal genomic space, fulfill their function without the need of protein cofactors, and most importantly are reprogrammable with respect to ligand selectivity and the RNA function to be regulated. Technologies that enable the generation of aptazymes to defined input ligands are of interest for the construction of biocomputing devices and biosensing applications. In this chapter we present a method that facilitates the in vivo screening of randomized pools of aptazymes in mammalian cells.

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540 Chemie

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Riboswitch, Aptazyme, Ribozyme, Biosensor, Synthetic biology

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ISO 690REHM, Charlotte, Benedikt KLAUSER, Jörg S. HARTIG, 2015. Engineering Aptazyme Switches for Conditional Gene Expression in Mammalian Cells Utilizing an In Vivo Screening Approach. In: PONCHON, Luc, ed.. RNA Scaffolds : Methods and Protocols. New York: Springer, 2015, pp. 127-140. Methods in Molecular Biology. 1316. ISBN 978-1-4939-2729-6. Available under: doi: 10.1007/978-1-4939-2730-2_11
BibTex
@incollection{Rehm2015Engin-33211,
  year={2015},
  doi={10.1007/978-1-4939-2730-2_11},
  title={Engineering Aptazyme Switches for Conditional Gene Expression in Mammalian Cells Utilizing an In Vivo Screening Approach},
  number={1316},
  isbn={978-1-4939-2729-6},
  publisher={Springer},
  address={New York},
  series={Methods in Molecular Biology},
  booktitle={RNA Scaffolds : Methods and Protocols},
  pages={127--140},
  editor={Ponchon, Luc},
  author={Rehm, Charlotte and Klauser, Benedikt and Hartig, Jörg S.}
}
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