Publikation:

Reversible, meniscus-free molecular combing of long-chain DNA

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Datum

2007

Autor:innen

Koota, Juha
Seuffert, Ina
Lehner, Roman

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URI (zitierfähiger Link)
http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-54458
DOI (zitierfähiger Link)
https://doi.org/10.1021/la7013122
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Attribution-NonCommercial-NoDerivs 2.0 Generic

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Open Access-Veröffentlichung
Open Access Green

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Biologie: Publikationen
Physik: Publikationen
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Published

Erschienen in

Langmuir. 2007, 23(18), pp. 9365-9368. ISSN 0743-7463. eISSN 1520-5827. Available under: doi: 10.1021/la7013122

Zusammenfassung

We introduce a method for reversibly orienting long-chain DNA on solid hydrophilic substrates without a fluid meniscus. End-tethered ì-DNA mushrooms are elongated by a hydrodynamic flow in the presence of trivalent cations, resulting in electrostatic adsorption of the extended DNA to the surface. By complexation of the cations the part of the DNA which is unspecifically bound to the surface desorbs quantitatively, and the mushroom conformation is restored. With the use of multiple deposition-combing steps, combined with a final desorption step, tethering densities higher than attainable with single deposition steps can be obtained.

Zusammenfassung in einer weiteren Sprache

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530 Physik

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ISO 690KOOTA, Juha, Ina SEUFFERT, Roman LEHNER, Jun LI, Thomas GISLER, 2007. Reversible, meniscus-free molecular combing of long-chain DNA. In: Langmuir. 2007, 23(18), pp. 9365-9368. ISSN 0743-7463. eISSN 1520-5827. Available under: doi: 10.1021/la7013122
BibTex
@article{Koota2007Rever-4892,
  year={2007},
  doi={10.1021/la7013122},
  title={Reversible, meniscus-free molecular combing of long-chain DNA},
  number={18},
  volume={23},
  issn={0743-7463},
  journal={Langmuir},
  pages={9365--9368},
  author={Koota, Juha and Seuffert, Ina and Lehner, Roman and Li, Jun and Gisler, Thomas}
}
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