Publikation:

Purification of 6-pyruvoyl-tetrahydropterin synthase from human liver

Vorschaubild

Dateien

Purification_of_6_pyruvoyl_tetrahydropterin_synthase_from_human_liver.pdf
Purification_of_6_pyruvoyl_tetrahydropterin_synthase_from_human_liver.pdfGröße: 663.38 KBDownloads: 678

Datum

1986

Autor:innen

Takikawa, Shin-Ichiro
Curtius, Hans-Christoph
Redweik, Udo

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

URI (zitierfähiger Link)
http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-56661
DOI (zitierfähiger Link)
https://doi.org/10.1016/S0006-291X(86)80468-5
ArXiv-ID

Internationale Patentnummer

Link zur Lizenz
Attribution-NonCommercial-NoDerivs 2.0 Generic

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung
Open Access Green

Sammlungen

Biologie: Publikationen
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

Biochemical and Biophysical Research Communications. 1986, 134(2), pp. 646-651. ISSN 0006-291X. Available under: doi: 10.1016/S0006-291X(86)80468-5

Zusammenfassung

The enzyme which catalyzes the first step in the conversion of dihydroneopterin triphosphate to tetrahydrobiopterin has been purified approx. 40,000-fold from human liver to apparent homogeneity. The enzyme has a native molecular weight of ~83,000 and consists of four identical subunits, each of which has a molecular weight of ~19,000. It contains carbohydrates and is remarkably stable to heat treatment. In the presence of purified sepiapterin reductase, Mg2+, and NADPH, this enzyme catalyzes efficiently the formation of tetrahydrobiopterin from dihydroneopterin triphosphate. This indicates that these two proteins are sufficient for the overall conversion.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690TAKIKAWA, Shin-Ichiro, Hans-Christoph CURTIUS, Udo REDWEIK, Sandro GHISLA, 1986. Purification of 6-pyruvoyl-tetrahydropterin synthase from human liver. In: Biochemical and Biophysical Research Communications. 1986, 134(2), pp. 646-651. ISSN 0006-291X. Available under: doi: 10.1016/S0006-291X(86)80468-5
BibTex
@article{Takikawa1986Purif-8308,
  year={1986},
  doi={10.1016/S0006-291X(86)80468-5},
  title={Purification of 6-pyruvoyl-tetrahydropterin synthase from human liver},
  number={2},
  volume={134},
  issn={0006-291X},
  journal={Biochemical and Biophysical Research Communications},
  pages={646--651},
  author={Takikawa, Shin-Ichiro and Curtius, Hans-Christoph and Redweik, Udo and Ghisla, Sandro}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/8308">
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8308"/>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:42:34Z</dcterms:available>
    <dc:contributor>Ghisla, Sandro</dc:contributor>
    <dc:creator>Curtius, Hans-Christoph</dc:creator>
    <dcterms:title>Purification of 6-pyruvoyl-tetrahydropterin synthase from human liver</dcterms:title>
    <dc:creator>Redweik, Udo</dc:creator>
    <dc:format>application/pdf</dc:format>
    <dc:language>eng</dc:language>
    <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:42:34Z</dc:date>
    <dc:creator>Takikawa, Shin-Ichiro</dc:creator>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8308/1/Purification_of_6_pyruvoyl_tetrahydropterin_synthase_from_human_liver.pdf"/>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:issued>1986</dcterms:issued>
    <dc:contributor>Redweik, Udo</dc:contributor>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/>
    <dcterms:abstract xml:lang="eng">The enzyme which catalyzes the first step in the conversion of dihydroneopterin triphosphate to tetrahydrobiopterin has been purified approx. 40,000-fold from human liver to apparent homogeneity. The enzyme has a native molecular weight of ~83,000 and consists of four identical subunits, each of which has a molecular weight of ~19,000. It contains carbohydrates and is remarkably stable to heat treatment. In the presence of purified sepiapterin reductase, Mg2+, and NADPH, this enzyme catalyzes efficiently the formation of tetrahydrobiopterin from dihydroneopterin triphosphate. This indicates that these two proteins are sufficient for the overall conversion.</dcterms:abstract>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Takikawa, Shin-Ichiro</dc:contributor>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8308/1/Purification_of_6_pyruvoyl_tetrahydropterin_synthase_from_human_liver.pdf"/>
    <dc:creator>Ghisla, Sandro</dc:creator>
    <dc:contributor>Curtius, Hans-Christoph</dc:contributor>
    <dcterms:bibliographicCitation>First publ. in: Biochemical and Biophysical Research Communications 134 (1986), 2, pp. 646-651</dcterms:bibliographicCitation>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
Begutachtet
Diese Publikation teilen