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Proteomic responses in the human dopaminergic LUHMES cell line to imidacloprid and its metabolites imidacloprid-olefin and desnitro-imidacloprid

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2023

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Sinclair, Patricia
Hakeem, Julia
Kumar, Sreehari G.
Loser, Dominik
Dixit, Kushan
Kraushaar, Udo
Kabbani, Nadine

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Pesticide Biochemistry and Physiology. Elsevier. 2023, 194, 105473. ISSN 0048-3575. eISSN 1095-9939. Available under: doi: 10.1016/j.pestbp.2023.105473

Zusammenfassung

Neonicotinoids (neonics) are amongst the most commonly used class of pesticides globally. In the United States, imidacloprid (IMI) is extensively used for agriculture and in other common applications such as house-hold pest control. Regular exposure to IMI, and several of its known metabolites including IMI-olefin and desnitro-imidacloprid (DN-IMI), has been shown to be harmful to many organisms including mammals, birds, and fish. Studies show that neonics bind human nicotinicacetylcholine receptors (nAChRs) and cause cellular toxicity. In the dopaminergic Lund human mesencephalic (LUHMES) cell line, IMI and other neonics (10–100 μM) have been recently shown to activate intracellular calcium signaling through nAChRs. Thus, we examined proteomic responses of LUHMES cells to a 48-h treatment with 50 μM IMI, IMI-olefin, or DN-IMI. Our findings show differential effects of these neonics on cellular protein expression. Bioinformatic analysis of significantly altered proteins indicates an effect of IMI, IMI-olefin, and DN-IMI on protein synthesis and ribosomal function. These findings suggest a role for protein synthesis and transcriptional regulation in neonic-mediated dopaminergic neurotoxicity.

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570 Biowissenschaften, Biologie

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ISO 690SINCLAIR, Patricia, Julia HAKEEM, Sreehari G. KUMAR, Dominik LOSER, Kushan DIXIT, Marcel LEIST, Udo KRAUSHAAR, Nadine KABBANI, 2023. Proteomic responses in the human dopaminergic LUHMES cell line to imidacloprid and its metabolites imidacloprid-olefin and desnitro-imidacloprid. In: Pesticide Biochemistry and Physiology. Elsevier. 2023, 194, 105473. ISSN 0048-3575. eISSN 1095-9939. Available under: doi: 10.1016/j.pestbp.2023.105473
BibTex
@article{Sinclair2023-08Prote-67095,
  year={2023},
  doi={10.1016/j.pestbp.2023.105473},
  title={Proteomic responses in the human dopaminergic LUHMES cell line to imidacloprid and its metabolites imidacloprid-olefin and desnitro-imidacloprid},
  volume={194},
  issn={0048-3575},
  journal={Pesticide Biochemistry and Physiology},
  author={Sinclair, Patricia and Hakeem, Julia and Kumar, Sreehari G. and Loser, Dominik and Dixit, Kushan and Leist, Marcel and Kraushaar, Udo and Kabbani, Nadine},
  note={Article Number: 105473}
}
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