Intracellular Protein-Lipid Interactions Studied by Rapid-Scan Electron Paramagnetic Resonance Spectroscopy

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The journal of physical chemistry letters. American Chemical Society (ACS). 2021, 12(9), pp. 2471-2475. eISSN 1948-7185. Available under: doi: 10.1021/acs.jpclett.0c03583
Zusammenfassung

Protein-membrane interactions play key roles in essential cellular processes; studying these interactions in the cell is a challenging task of modern biophysical chemistry. A prominent example is the interaction of human α-synuclein (αS) with negatively charged membranes. It has been well-studied in vitro, but in spite of the huge amount of lipid membranes in the crowded environment of biological cells, to date, no interactions have been detected in cells. Here, we use rapid-scan (RS) electron paramagnetic resonance (EPR) spectroscopy to study αS interactions with negatively charged vesicles in vitro and upon transfection of the protein and lipid vesicles into model cells, i.e., oocytes of Xenopus laevis. We show that protein-vesicle interactions are reflected in RS spectra in vitro and in cells, which enables time-resolved monitoring of protein-membrane interaction upon transfection into cells. Our data suggest binding of a small fraction of αS to endogenous membranes.

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540 Chemie
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Vesicles, Lipids, Membranes, Electron paramagnetic resonance spectroscopy, Spectroscopy
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ISO 690BRAUN, Theresa S., Juliane STEHLE, Sylwia KACPRZAK, Patrick CARL, Peter HÖFER, Vinod SUBRAMANIAM, Malte DRESCHER, 2021. Intracellular Protein-Lipid Interactions Studied by Rapid-Scan Electron Paramagnetic Resonance Spectroscopy. In: The journal of physical chemistry letters. American Chemical Society (ACS). 2021, 12(9), pp. 2471-2475. eISSN 1948-7185. Available under: doi: 10.1021/acs.jpclett.0c03583
BibTex
@article{Braun2021-03-11Intra-53365,
  year={2021},
  doi={10.1021/acs.jpclett.0c03583},
  title={Intracellular Protein-Lipid Interactions Studied by Rapid-Scan Electron Paramagnetic Resonance Spectroscopy},
  number={9},
  volume={12},
  journal={The journal of physical chemistry letters},
  pages={2471--2475},
  author={Braun, Theresa S. and Stehle, Juliane and Kacprzak, Sylwia and Carl, Patrick and Höfer, Peter and Subramaniam, Vinod and Drescher, Malte}
}
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