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Structural and mechanistic insights into Quinolone Synthase to address its functional promiscuity

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2024

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Vijayanathan, Mallika
Vadakkepat, Abhinav Koyamangalath
Mahendran, Kozhinjampara R.
Frandsen, Kristian E. H.
Bandyopadhyay, Debashree
Pillai, M. Radhakrishna
Soniya, Eppurath Vasudevan

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Communications Biology. Springer. 2024, 7(1), 566. eISSN 2399-3642. Verfügbar unter: doi: 10.1038/s42003-024-06152-2

Zusammenfassung

Quinolone synthase from Aegle marmelos (AmQNS) is a type III polyketide synthase that yields therapeutically effective quinolone and acridone compounds. Addressing the structural and molecular underpinnings of AmQNS and its substrate interaction in terms of its high selectivity and specificity can aid in the development of numerous novel compounds. This paper presents a high-resolution AmQNS crystal structure and explains its mechanistic role in synthetic selectivity. Additionally, we provide a model framework to comprehend structural constraints on ketide insertion and postulate that AmQNS’s steric and electrostatic selectivity plays a role in its ability to bind to various core substrates, resulting in its synthetic diversity. AmQNS prefers quinolone synthesis and can accommodate large substrates because of its wide active site entrance. However, our research suggests that acridone is exclusively synthesized in the presence of high malonyl-CoA concentrations. Potential implications of functionally relevant residue mutations were also investigated, which will assist in harnessing the benefits of mutations for targeted polyketide production. The pharmaceutical industry stands to gain from these findings as they expand the pool of potential drug candidates, and these methodologies can also be applied to additional promising enzymes.

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570 Biowissenschaften, Biologie

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Biotechnology, X-ray crystallography

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ISO 690VIJAYANATHAN, Mallika, Abhinav Koyamangalath VADAKKEPAT, Kozhinjampara R. MAHENDRAN, Abdoallah SHARAF, Kristian E. H. FRANDSEN, Debashree BANDYOPADHYAY, M. Radhakrishna PILLAI, Eppurath Vasudevan SONIYA, 2024. Structural and mechanistic insights into Quinolone Synthase to address its functional promiscuity. In: Communications Biology. Springer. 2024, 7(1), 566. eISSN 2399-3642. Verfügbar unter: doi: 10.1038/s42003-024-06152-2
BibTex
@article{Vijayanathan2024-05-14Struc-71868,
  year={2024},
  doi={10.1038/s42003-024-06152-2},
  title={Structural and mechanistic insights into Quinolone Synthase to address its functional promiscuity},
  number={1},
  volume={7},
  journal={Communications Biology},
  author={Vijayanathan, Mallika and Vadakkepat, Abhinav Koyamangalath and Mahendran, Kozhinjampara R. and Sharaf, Abdoallah and Frandsen, Kristian E. H. and Bandyopadhyay, Debashree and Pillai, M. Radhakrishna and Soniya, Eppurath Vasudevan},
  note={Article Number: 566}
}
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