Genetically Encoded Spin Labels for In Vitro and In-Cell EPR Studies of Native Proteins

Lade...
Vorschaubild
Dateien
Zu diesem Dokument gibt es keine Dateien.
Datum
2015
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
URI (zitierfähiger Link)
ArXiv-ID
Internationale Patentnummer
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Core Facility der Universität Konstanz
Gesperrt bis
Titel in einer weiteren Sprache
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Publikationstyp
Beitrag zu einem Sammelband
Publikationsstatus
Published
Erschienen in
QIN, Peter Z., ed. and others. Electron Paramagnetic Resonance Investigations of Biological Systems by Using Spin Labels, Spin Probes, and Intrinsic Metal Ions ; Part A. Amsterdam: Elsevier, 2015, pp. 483-502. Methods in Enzymology. 563. ISBN 978-0-12-802834-6. Available under: doi: 10.1016/bs.mie.2015.05.023
Zusammenfassung

Electron paramagnetic resonance (EPR) spectroscopy in combination with site-directed spin labeling (SDSL) is a powerful approach to study the structure, dynamics, and interactions of proteins. The genetic encoding of the noncanonical amino acid spin-labeled lysine 1 (SLK-1) eliminates the need for any chemical labeling steps in SDSL-EPR studies and enables the investigation of native, endogenous proteins with minimal structural perturbation, and without the need to create unique reactive sites for chemical labeling. We report detailed experimental procedures for the efficient synthesis of SLK-1, the expression and purification of SLK-1-containing proteins under conditions that ensure maximal integrity of the nitroxide radical moiety, and procedures for intramolecular EPR distance measurements in proteins by double electron-electron resonance.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
540 Chemie
Schlagwörter
Noncanonical amino acids, Genetic encoding, EPR spectroscopy, Site-directed spin labeling, In-cell EPR, DEER, Thioredoxin
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690SCHMIDT, Moritz J., Artem FEDOSEEV, Daniel SUMMERER, Malte DRESCHER, 2015. Genetically Encoded Spin Labels for In Vitro and In-Cell EPR Studies of Native Proteins. In: QIN, Peter Z., ed. and others. Electron Paramagnetic Resonance Investigations of Biological Systems by Using Spin Labels, Spin Probes, and Intrinsic Metal Ions ; Part A. Amsterdam: Elsevier, 2015, pp. 483-502. Methods in Enzymology. 563. ISBN 978-0-12-802834-6. Available under: doi: 10.1016/bs.mie.2015.05.023
BibTex
@incollection{Schmidt2015Genet-33218,
  year={2015},
  doi={10.1016/bs.mie.2015.05.023},
  title={Genetically Encoded Spin Labels for In Vitro and In-Cell EPR Studies of Native Proteins},
  number={563},
  isbn={978-0-12-802834-6},
  publisher={Elsevier},
  address={Amsterdam},
  series={Methods in Enzymology},
  booktitle={Electron Paramagnetic Resonance Investigations of Biological Systems by Using Spin Labels, Spin Probes, and Intrinsic Metal Ions ; Part A},
  pages={483--502},
  editor={Qin, Peter Z.},
  author={Schmidt, Moritz J. and Fedoseev, Artem and Summerer, Daniel and Drescher, Malte}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/33218">
    <dc:contributor>Summerer, Daniel</dc:contributor>
    <dc:contributor>Drescher, Malte</dc:contributor>
    <dc:language>eng</dc:language>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/52"/>
    <dc:contributor>Fedoseev, Artem</dc:contributor>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-03-03T14:20:29Z</dcterms:available>
    <dcterms:abstract xml:lang="eng">Electron paramagnetic resonance (EPR) spectroscopy in combination with site-directed spin labeling (SDSL) is a powerful approach to study the structure, dynamics, and interactions of proteins. The genetic encoding of the noncanonical amino acid spin-labeled lysine 1 (SLK-1) eliminates the need for any chemical labeling steps in SDSL-EPR studies and enables the investigation of native, endogenous proteins with minimal structural perturbation, and without the need to create unique reactive sites for chemical labeling. We report detailed experimental procedures for the efficient synthesis of SLK-1, the expression and purification of SLK-1-containing proteins under conditions that ensure maximal integrity of the nitroxide radical moiety, and procedures for intramolecular EPR distance measurements in proteins by double electron-electron resonance.</dcterms:abstract>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-03-03T14:20:29Z</dc:date>
    <dcterms:title>Genetically Encoded Spin Labels for In Vitro and In-Cell EPR Studies of Native Proteins</dcterms:title>
    <dc:creator>Drescher, Malte</dc:creator>
    <dc:creator>Fedoseev, Artem</dc:creator>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/33218"/>
    <dc:creator>Schmidt, Moritz J.</dc:creator>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dcterms:issued>2015</dcterms:issued>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:contributor>Schmidt, Moritz J.</dc:contributor>
    <dc:creator>Summerer, Daniel</dc:creator>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/52"/>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Diese Publikation teilen