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Characterizing Host Receptor Recognition by Individual Bacterial Pathogens

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2009

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Kuespert, Katharina

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https://doi.org/10.1007/978-1-59745-204-5_5
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RUPP, Steffen, ed. and others. Host-pathogen interactions : methods and protocols. Totowa, NJ: Humana Press, 2009, pp. 57-65. Methods in Molecular Biology. 470. ISBN 978-1-58829-886-7. Available under: doi: 10.1007/978-1-59745-204-5_5

Zusammenfassung

A critical determinant of host range and specificity relies on the ability of pathogenic bacteria to recognize eukaryotic cell surface molecules via specialized adhesins. The specific adhesin-receptor interaction allows pathogens to tightly bind to their target cells, thereby facilitating the colonization of host tissues. Therefore, the identification and characterization of bacterial adhesins is a major topic in infection biology. This chapter focuses on a rapid and simple method for the analysis of adhesin-receptor interactions that permits the characterization of receptor binding properties at the level of single bacteria. Accordingly, this methodological approach is ideally suited for the analysis of adhesins expressed in a phase-variable manner and for the study of heterogeneous bacterial populations. Besides focusing on the receptor-binding assay, this chapter describes the production of fluorescence-tagged soluble host receptor domains required for conducting this assay.

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570 Biowissenschaften, Biologie

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ISO 690KUESPERT, Katharina, Christof R. HAUCK, 2009. Characterizing Host Receptor Recognition by Individual Bacterial Pathogens. In: RUPP, Steffen, ed. and others. Host-pathogen interactions : methods and protocols. Totowa, NJ: Humana Press, 2009, pp. 57-65. Methods in Molecular Biology. 470. ISBN 978-1-58829-886-7. Available under: doi: 10.1007/978-1-59745-204-5_5
BibTex
@incollection{Kuespert2009Chara-40582,
  year={2009},
  doi={10.1007/978-1-59745-204-5_5},
  title={Characterizing Host Receptor Recognition by Individual Bacterial Pathogens},
  number={470},
  isbn={978-1-58829-886-7},
  publisher={Humana Press},
  address={Totowa, NJ},
  series={Methods in Molecular Biology},
  booktitle={Host-pathogen interactions : methods and protocols},
  pages={57--65},
  editor={Rupp, Steffen},
  author={Kuespert, Katharina and Hauck, Christof R.}
}
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