Publikation:

Cascade of Caspase Activation in Potassium-Deprived Cerebellar Granule Neurons : Targets for Treatment with Peptide and Protein Inhibitors of Apoptosis

Lade...
Vorschaubild

Dateien

Zu diesem Dokument gibt es keine Dateien.

Datum

2001

Autor:innen

Gerhardt, Ellen
Kügler, Sebastian
Beier, Christoph
Berliocchi, Laura
Volbracht, Christiane
Weller, Michael
Bähr, Mathias
Schulz, Jörg B.

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

URI (zitierfähiger Link)
ArXiv-ID

Internationale Patentnummer

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

Molecular and Cellular Neuroscience. Elsevier. 2001, 17(4), pp. 717-731. ISSN 1044-7431. eISSN 1095-9327. Available under: doi: 10.1006/mcne.2001.0962

Zusammenfassung

Cerebellar granule neurons (CGN) cultured in the presence of serum and depolarizing potassium concentrations undergo apoptosis when switched to serum-free medium containing physiological potassium concentrations. Here we show that processing of the key protease, caspase-3, depends on the activation of caspase-9, but not of caspase-8. Selective peptide inhibitors of caspase-9 block processing of caspase-3 and caspase-8 and inhibit apoptosis, whereas a selective inhibitor of caspase-8 blocks neither processing of caspase-3 nor cell death. The data obtained with peptide inhibitors were confirmed by adenovirally mediated ectopic expression of the cytokine response modifier A (crmA), the baculovirus protein p35, and the X chromosome-linked inhibitor of apoptosis (XIAP). Further, caspase-8-activating death receptors do not mediate apoptosis in CGN and potassium withdrawal-induced apoptosis evolves unaltered in gld or lpr mice, which harbor mutations in the CD95/CD95 ligand system. Thus, neuronal apoptosis triggered by potassium deprivation is death receptor-independent but involves the mitochondrial pathway of caspase activation.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690GERHARDT, Ellen, Sebastian KÜGLER, Marcel LEIST, Christoph BEIER, Laura BERLIOCCHI, Christiane VOLBRACHT, Michael WELLER, Mathias BÄHR, Pierluigi NICOTERA, Jörg B. SCHULZ, 2001. Cascade of Caspase Activation in Potassium-Deprived Cerebellar Granule Neurons : Targets for Treatment with Peptide and Protein Inhibitors of Apoptosis. In: Molecular and Cellular Neuroscience. Elsevier. 2001, 17(4), pp. 717-731. ISSN 1044-7431. eISSN 1095-9327. Available under: doi: 10.1006/mcne.2001.0962
BibTex
@article{Gerhardt2001-04Casca-51267,
  year={2001},
  doi={10.1006/mcne.2001.0962},
  title={Cascade of Caspase Activation in Potassium-Deprived Cerebellar Granule Neurons : Targets for Treatment with Peptide and Protein Inhibitors of Apoptosis},
  number={4},
  volume={17},
  issn={1044-7431},
  journal={Molecular and Cellular Neuroscience},
  pages={717--731},
  author={Gerhardt, Ellen and Kügler, Sebastian and Leist, Marcel and Beier, Christoph and Berliocchi, Laura and Volbracht, Christiane and Weller, Michael and Bähr, Mathias and Nicotera, Pierluigi and Schulz, Jörg B.}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/51267">
    <dc:creator>Nicotera, Pierluigi</dc:creator>
    <dcterms:abstract xml:lang="eng">Cerebellar granule neurons (CGN) cultured in the presence of serum and depolarizing potassium concentrations undergo apoptosis when switched to serum-free medium containing physiological potassium concentrations. Here we show that processing of the key protease, caspase-3, depends on the activation of caspase-9, but not of caspase-8. Selective peptide inhibitors of caspase-9 block processing of caspase-3 and caspase-8 and inhibit apoptosis, whereas a selective inhibitor of caspase-8 blocks neither processing of caspase-3 nor cell death. The data obtained with peptide inhibitors were confirmed by adenovirally mediated ectopic expression of the cytokine response modifier A (crmA), the baculovirus protein p35, and the X chromosome-linked inhibitor of apoptosis (XIAP). Further, caspase-8-activating death receptors do not mediate apoptosis in CGN and potassium withdrawal-induced apoptosis evolves unaltered in gld or lpr mice, which harbor mutations in the CD95/CD95 ligand system. Thus, neuronal apoptosis triggered by potassium deprivation is death receptor-independent but involves the mitochondrial pathway of caspase activation.</dcterms:abstract>
    <dc:creator>Volbracht, Christiane</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Gerhardt, Ellen</dc:contributor>
    <dc:creator>Schulz, Jörg B.</dc:creator>
    <dcterms:issued>2001-04</dcterms:issued>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2020-10-09T06:58:41Z</dc:date>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dc:rights>terms-of-use</dc:rights>
    <dc:creator>Weller, Michael</dc:creator>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:creator>Leist, Marcel</dc:creator>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/51267"/>
    <dc:creator>Bähr, Mathias</dc:creator>
    <dc:contributor>Schulz, Jörg B.</dc:contributor>
    <dcterms:title>Cascade of Caspase Activation in Potassium-Deprived Cerebellar Granule Neurons : Targets for Treatment with Peptide and Protein Inhibitors of Apoptosis</dcterms:title>
    <dc:contributor>Volbracht, Christiane</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:contributor>Beier, Christoph</dc:contributor>
    <dc:creator>Beier, Christoph</dc:creator>
    <dc:contributor>Leist, Marcel</dc:contributor>
    <dc:language>eng</dc:language>
    <dc:contributor>Berliocchi, Laura</dc:contributor>
    <dc:creator>Berliocchi, Laura</dc:creator>
    <dc:contributor>Bähr, Mathias</dc:contributor>
    <dc:contributor>Kügler, Sebastian</dc:contributor>
    <dc:creator>Kügler, Sebastian</dc:creator>
    <dc:contributor>Weller, Michael</dc:contributor>
    <dc:creator>Gerhardt, Ellen</dc:creator>
    <dc:contributor>Nicotera, Pierluigi</dc:contributor>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2020-10-09T06:58:41Z</dcterms:available>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
Begutachtet
Ja
Diese Publikation teilen