Publikation: Specific local induction of DNA strand breaks by infrared multi-photon absorption
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2010
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Nucleic Acids Research. 2010, 38(3), e14. Available under: doi: 10.1093/nar/gkp932
Zusammenfassung
Highly confined DNA damage by femtosecond laser irradiation currently arises as a powerful tool to understand DNA repair in live cells as a function of space and time. However, the specificity with respect to damage type is limited. Here, we present an irradiation procedure based on a widely tunable Er/Yb : fiber femtosecond laser source that favors the formation of DNA strand breaks over that of UV photoproducts by more than one order of magnitude. We explain this selectivity with the different power dependence of the reactions generating strand breaks, mainly involving reactive radical intermediates, and the direct photochemical process leading to UV-photoproducts. Thus, localized multi-photon excitation with a wavelength longer than 1 km allows for the selective production of DNA strand breaks at submicrometer spatial resolution in the absence of photosensitizers.
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TRÄUTLEIN, Daniel, Martin DEIBLER, Alfred LEITENSTORFER, Elisa FERRANDO-MAY, 2010. Specific local induction of DNA strand breaks by infrared multi-photon absorption. In: Nucleic Acids Research. 2010, 38(3), e14. Available under: doi: 10.1093/nar/gkp932BibTex
@article{Trautlein2010Speci-13483,
year={2010},
doi={10.1093/nar/gkp932},
title={Specific local induction of DNA strand breaks by infrared multi-photon absorption},
number={3},
volume={38},
journal={Nucleic Acids Research},
author={Träutlein, Daniel and Deibler, Martin and Leitenstorfer, Alfred and Ferrando-May, Elisa},
note={Article Number: e14}
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<dcterms:abstract xml:lang="eng">Highly confined DNA damage by femtosecond laser irradiation currently arises as a powerful tool to understand DNA repair in live cells as a function of space and time. However, the specificity with respect to damage type is limited. Here, we present an irradiation procedure based on a widely tunable Er/Yb : fiber femtosecond laser source that favors the formation of DNA strand breaks over that of UV photoproducts by more than one order of magnitude. We explain this selectivity with the different power dependence of the reactions generating strand breaks, mainly involving reactive radical intermediates, and the direct photochemical process leading to UV-photoproducts. Thus, localized multi-photon excitation with a wavelength longer than 1 km allows for the selective production of DNA strand breaks at submicrometer spatial resolution in the absence of photosensitizers.</dcterms:abstract>
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