Membrane traffic and Ca2+ signals in ciliates

Lade...
Vorschaubild
Dateien
Plattner_2-1odm54lxr18e11.pdf
Plattner_2-1odm54lxr18e11.pdfGröße: 3.27 MBDownloads: 205
Datum
2022
Autor:innen
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
DOI (zitierfähiger Link)
ArXiv-ID
Internationale Patentnummer
Link zur Lizenz
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Open Access Hybrid
Sammlungen
Core Facility der Universität Konstanz
Gesperrt bis
Titel in einer weiteren Sprache
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
Zusammenfassung

A Paramecium cell has as many types of membrane interactions as mammalian cells, as established with monoclonal antibodies by R. Allen and A. Fok. Since then, we have identified key players, such as SNARE proteins, Ca2+-regulating proteins, including Ca2+-channels, Ca2+-pumps, Ca2+-binding proteins of different affinity, etc., at the molecular level, probed their function and localized them at the light and electron microscopy level. SNARE proteins, in conjunction with a synaptotagmin-like Ca2+-sensor protein, mediate membrane fusion. This interaction is additionally regulated by monomeric GTPases whose spectrum in Tetrahymena and Paramecium has been established by A. Turkewitz. As known from mammalian cells, GTPases are activated on membranes in conjunction with lumenal acidification by an H+-ATPase. For these complex molecules, we found in Paramecium an unsurpassed number of 17 a-subunit paralogs which connect the polymeric head and basis part, V1 and V0. (This multitude may reflect different local functional requirements.) Together with plasmalemmal Ca2+-influx channels, locally enriched intracellular InsP3-type (InsP3R, mainly in osmoregulatory system) and ryanodine receptor-like Ca2+-release channels (ryanodine receptor-like proteins, RyR-LP), this complexity mediates Ca2+ signals for most flexible local membrane-to-membrane interactions. As we found, the latter channel types miss a substantial portion of the N-terminal part. Caffeine and 4-chloro-meta-cresol (the agent used to probe mutations of RyRs in man during surgery in malignant insomnia patients) initiate trichocyst exocytosis by activating Ca2+-release channels type CRC-IV in the peripheral part of alveolar sacs. This is superimposed by Ca2+-influx, that is, a mechanism called “store-operated Ca2+-entry” (SOCE). For the majority of key players, we have mapped paralogs throughout the Paramecium cell, with features in common or at variance in the different organelles participating in vesicle trafficking. Local values of free Ca2+-concentration, [Ca2+]i, and their change, for example, upon exocytosis stimulation, have been registered by flurochromes and chelator effects. In parallel, we have registered release of Ca2+ from alveolar sacs by quenched-flow analysis combined with cryofixation and X-ray microanalysis.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690PLATTNER, Helmut, 2022. Membrane traffic and Ca2+ signals in ciliates. In: The Journal of Eukaryotic Microbiology. Wiley. 2022, 69(5), e12895. ISSN 1066-5234. eISSN 1550-7408. Available under: doi: 10.1111/jeu.12895
BibTex
@article{Plattner2022-09Membr-56960,
  year={2022},
  doi={10.1111/jeu.12895},
  title={Membrane traffic and Ca<sup>2+</sup> signals in ciliates},
  number={5},
  volume={69},
  issn={1066-5234},
  journal={The Journal of Eukaryotic Microbiology},
  author={Plattner, Helmut},
  note={Article Number: e12895}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/56960">
    <dc:contributor>Plattner, Helmut</dc:contributor>
    <dcterms:issued>2022-09</dcterms:issued>
    <dcterms:title>Membrane traffic and Ca&lt;sup&gt;2+&lt;/sup&gt; signals in ciliates</dcterms:title>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/56960"/>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by/4.0/"/>
    <dc:creator>Plattner, Helmut</dc:creator>
    <dc:language>eng</dc:language>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-03-23T09:55:35Z</dc:date>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/56960/1/Plattner_2-1odm54lxr18e11.pdf"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:rights>Attribution 4.0 International</dc:rights>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/56960/1/Plattner_2-1odm54lxr18e11.pdf"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:abstract xml:lang="eng">A Paramecium cell has as many types of membrane interactions as mammalian cells, as established with monoclonal antibodies by R. Allen and A. Fok. Since then, we have identified key players, such as SNARE proteins, Ca&lt;sup&gt;2+&lt;/sup&gt;-regulating proteins, including Ca&lt;sup&gt;2+&lt;/sup&gt;-channels, Ca&lt;sup&gt;2+&lt;/sup&gt;-pumps, Ca&lt;sup&gt;2+&lt;/sup&gt;-binding proteins of different affinity, etc., at the molecular level, probed their function and localized them at the light and electron microscopy level. SNARE proteins, in conjunction with a synaptotagmin-like Ca&lt;sup&gt;2+&lt;/sup&gt;-sensor protein, mediate membrane fusion. This interaction is additionally regulated by monomeric GTPases whose spectrum in Tetrahymena and Paramecium has been established by A. Turkewitz. As known from mammalian cells, GTPases are activated on membranes in conjunction with lumenal acidification by an H&lt;sup&gt;+&lt;/sup&gt;-ATPase. For these complex molecules, we found in Paramecium an unsurpassed number of 17 a-subunit paralogs which connect the polymeric head and basis part, V1 and V0. (This multitude may reflect different local functional requirements.) Together with plasmalemmal Ca&lt;sup&gt;2+&lt;/sup&gt;-influx channels, locally enriched intracellular InsP&lt;sub&gt;3&lt;/sub&gt;-type (InsP&lt;sub&gt;3&lt;/sub&gt;R, mainly in osmoregulatory system) and ryanodine receptor-like Ca&lt;sup&gt;2+&lt;/sup&gt;-release channels (ryanodine receptor-like proteins, RyR-LP), this complexity mediates Ca&lt;sup&gt;2+&lt;/sup&gt; signals for most flexible local membrane-to-membrane interactions. As we found, the latter channel types miss a substantial portion of the N-terminal part. Caffeine and 4-chloro-meta-cresol (the agent used to probe mutations of RyRs in man during surgery in malignant insomnia patients) initiate trichocyst exocytosis by activating Ca&lt;sup&gt;2+&lt;/sup&gt;-release channels type CRC-IV in the peripheral part of alveolar sacs. This is superimposed by Ca&lt;sup&gt;2+&lt;/sup&gt;-influx, that is, a mechanism called “store-operated Ca&lt;sup&gt;2+&lt;/sup&gt;-entry” (SOCE). For the majority of key players, we have mapped paralogs throughout the Paramecium cell, with features in common or at variance in the different organelles participating in vesicle trafficking. Local values of free Ca&lt;sup&gt;2+&lt;/sup&gt;-concentration, [Ca&lt;sup&gt;2+&lt;/sup&gt;]i, and their change, for example, upon exocytosis stimulation, have been registered by flurochromes and chelator effects. In parallel, we have registered release of Ca&lt;sup&gt;2+&lt;/sup&gt; from alveolar sacs by quenched-flow analysis combined with cryofixation and X-ray microanalysis.</dcterms:abstract>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-03-23T09:55:35Z</dcterms:available>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Begutachtet
Ja
Diese Publikation teilen