Publikation:

The Genetic Incorporation of a Distance Probe into Proteins in Escherichia coli

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12940summerer18.pdf
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2006

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Tsao, Meng-Lin
Ryu, Youngha
Schultz, Peter G.

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http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-129403
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https://doi.org/10.1021/ja058262u
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Chemie: Publikationen
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Published

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Journal of the American Chemical Society. 2006, 128(14), pp. 4572-4573. ISSN 0002-7863. eISSN 1520-5126. Available under: doi: 10.1021/ja058262u

Zusammenfassung

The unnatural amino acid p-nitrophenylalanine (pNO2-Phe) was genetically introduced into proteins in Escherichia coli in response to the amber nonsense codon with high fidelity and efficiency by means of an evolved tRNA/aminoacyl-tRNA synthetase pair from Methanocuccus jannaschii. It was shown that pNO2-Phe efficiently quenches the intrinsic fluorescence of Trp in a distance-dependent manner in a model GCN4 basic region leucine zipper (bZIP) protein. Thus, the pNO2-Phe/Trp pair should be a useful biophysical probe of protein structure and function.

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540 Chemie

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ISO 690TSAO, Meng-Lin, Daniel SUMMERER, Youngha RYU, Peter G. SCHULTZ, 2006. The Genetic Incorporation of a Distance Probe into Proteins in Escherichia coli. In: Journal of the American Chemical Society. 2006, 128(14), pp. 4572-4573. ISSN 0002-7863. eISSN 1520-5126. Available under: doi: 10.1021/ja058262u
BibTex
@article{Tsao2006Genet-9879,
  year={2006},
  doi={10.1021/ja058262u},
  title={The Genetic Incorporation of a Distance Probe into Proteins in Escherichia coli},
  number={14},
  volume={128},
  issn={0002-7863},
  journal={Journal of the American Chemical Society},
  pages={4572--4573},
  author={Tsao, Meng-Lin and Summerer, Daniel and Ryu, Youngha and Schultz, Peter G.}
}
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