Publikation: Scraping and Stapling of End-Grafted DNA Chains by a Bioadhesive Spreading Vesicle to Reveal Chain Internal Friction and Topological Complexity
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2010
Autor:innen
Nam, Gimoon
Hisette, Marie Laure
Sun, Yuting Liang
Johner, Albert
Thalmann, Fabrice
Schröder, André Pierre
Marques, Carlos Manuel
Lee, Nam-Kyung
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Physical Review Letters. 2010, 105(8). ISSN 0031-9007. Available under: doi: 10.1103/PhysRevLett.105.088101
Zusammenfassung
Stained end-grafted DNA molecules about 20 μm long are scraped away and stretched out by the spreading front of a bioadhesive vesicle. Tethered biotin ligands bind the vesicle bilayer to a streptavidin substrate, stapling the DNAs into frozen confinement paths. Image analysis of the stapled DNA gives access, within optical resolution, to the local stretching values of individual DNA molecules swept by the spreading front, and provides evidence of self-entanglements.
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NAM, Gimoon, Marie Laure HISETTE, Yuting Liang SUN, Thomas GISLER, Albert JOHNER, Fabrice THALMANN, André Pierre SCHRÖDER, Carlos Manuel MARQUES, Nam-Kyung LEE, 2010. Scraping and Stapling of End-Grafted DNA Chains by a Bioadhesive Spreading Vesicle to Reveal Chain Internal Friction and Topological Complexity. In: Physical Review Letters. 2010, 105(8). ISSN 0031-9007. Available under: doi: 10.1103/PhysRevLett.105.088101BibTex
@article{Nam2010Scrap-12457,
year={2010},
doi={10.1103/PhysRevLett.105.088101},
title={Scraping and Stapling of End-Grafted DNA Chains by a Bioadhesive Spreading Vesicle to Reveal Chain Internal Friction and Topological Complexity},
number={8},
volume={105},
issn={0031-9007},
journal={Physical Review Letters},
author={Nam, Gimoon and Hisette, Marie Laure and Sun, Yuting Liang and Gisler, Thomas and Johner, Albert and Thalmann, Fabrice and Schröder, André Pierre and Marques, Carlos Manuel and Lee, Nam-Kyung}
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<dcterms:abstract xml:lang="eng">Stained end-grafted DNA molecules about 20 μm long are scraped away and stretched out by the spreading front of a bioadhesive vesicle. Tethered biotin ligands bind the vesicle bilayer to a streptavidin substrate, stapling the DNAs into frozen confinement paths. Image analysis of the stapled DNA gives access, within optical resolution, to the local stretching values of individual DNA molecules swept by the spreading front, and provides evidence of self-entanglements.</dcterms:abstract>
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