Publikation: Biotransformation of Chloromethane to Methanethiol
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1993
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Environmental Science and Technology. 1993, 27(8), pp. 1577-1579. ISSN 0013-936X. eISSN 1520-5851. Available under: doi: 10.1021/es00045a012
Zusammenfassung
Monochloromethane (MeCl) was biotransformed by a mixed culture of anaerobic bacteria which had been enriched to utilize dichloromethane as the sole source of carbon and energy. Cells grown in dichloromethane-salts medium catalyzed the transformation, whereas sucrose-grown cells were inactive. MeCl disappeared almost totally, and the methyl group was recovered in a yield of 80-90% as methanethiol and traces of dimethyl sulfide, whose identities were confirmed by GC-MS. The methyl transfer was heat-labile, and free aquocobalamin did not promote the reaction, which was apparently enzymically catalyzed.
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570 Biowissenschaften, Biologie
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STROMEYER, Susanna A., Alasdair M. COOK, Thomas LEISINGER, 1993. Biotransformation of Chloromethane to Methanethiol. In: Environmental Science and Technology. 1993, 27(8), pp. 1577-1579. ISSN 0013-936X. eISSN 1520-5851. Available under: doi: 10.1021/es00045a012BibTex
@article{Stromeyer1993Biotr-7210,
year={1993},
doi={10.1021/es00045a012},
title={Biotransformation of Chloromethane to Methanethiol},
number={8},
volume={27},
issn={0013-936X},
journal={Environmental Science and Technology},
pages={1577--1579},
author={Stromeyer, Susanna A. and Cook, Alasdair M. and Leisinger, Thomas}
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<dcterms:abstract xml:lang="eng">Monochloromethane (MeCl) was biotransformed by a mixed culture of anaerobic bacteria which had been enriched to utilize dichloromethane as the sole source of carbon and energy. Cells grown in dichloromethane-salts medium catalyzed the transformation, whereas sucrose-grown cells were inactive. MeCl disappeared almost totally, and the methyl group was recovered in a yield of 80-90% as methanethiol and traces of dimethyl sulfide, whose identities were confirmed by GC-MS. The methyl transfer was heat-labile, and free aquocobalamin did not promote the reaction, which was apparently enzymically catalyzed.</dcterms:abstract>
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