Publikation: Effects of antiretroviral drugs on human immunodeficiency virus type 1-induced CD4(+) T-cell death
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Apoptosis of peripheral blood T cells plays an important role in the pathogenesis of human immunodeficiency virus (HIV) infection. In this study, we found that HIV type 1 (HIV-1) primes CD4+ T cells from healthy donors for apoptosis, which occurs after CD95 ligation or CD3-T-cell receptor (TCR) stimulation. CD95-mediated death did not depend on CD4 T-cell infection, since it occurred in the presence of the reverse transcriptase inhibitor didanosine (ddI). In contrast, apoptosis induced by productive infection (CD3-TCR stimulation) is prevented by both CD95 decoy receptor and ddI. Our data suggest that HIV-1 triggers at least two distinct death pathways: a CD95-dependent pathway that does not require viral replication and a viral replication-mediated cell death independent of the CD95 pathway. Further experiments indicated that saquinavir, a protease inhibitor, at a 0.2 µM concentration, decreased HIV-mediated CD95 expression and thus cell death, which is independent of its role in inhibiting viral replication. However, treatment of peripheral blood mononuclear cells from healthy donors with a higher concentration (10 µM) of an HIV protease inhibitor, saquinavir or indinavir, induced both a loss in mitochondrial membrane potential ({Delta}{Psi}m) and cell death. Thus, protease inhibitors have the potential for both beneficial and detrimental effects on CD4+ T cells independent of their antiretroviral effects.
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ESTAQUIER, Jérôme, Jean-Daniel LELIÈVRE, Frédéric PETIT, Thomas BRUNNER, Laure MOUTOUH-DE PARSEVAL, Douglas D. RICHMAN, Jean Claude AMEISEN, Jacques CORBEIL, 2002. Effects of antiretroviral drugs on human immunodeficiency virus type 1-induced CD4(+) T-cell death. In: Journal of Virology. 2002, 76(12), pp. 5966-5973. ISSN 0022-538X. Available under: doi: 10.1128/JVI.76.12.5966-5973.2002BibTex
@article{Estaquier2002Effec-14291, year={2002}, doi={10.1128/JVI.76.12.5966-5973.2002}, title={Effects of antiretroviral drugs on human immunodeficiency virus type 1-induced CD4(+) T-cell death}, number={12}, volume={76}, issn={0022-538X}, journal={Journal of Virology}, pages={5966--5973}, author={Estaquier, Jérôme and Lelièvre, Jean-Daniel and Petit, Frédéric and Brunner, Thomas and Moutouh-de Parseval, Laure and Richman, Douglas D. and Ameisen, Jean Claude and Corbeil, Jacques} }
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