Publikation: Copper-induced oligomerization of peptides : a model study
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2007
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European Journal of Mass Spectrometry. 2007, 13(5), pp. 331-337. ISSN 1356-1049. Available under: doi: 10.1255/ejms.889
Zusammenfassung
In this work, copper-binding of the tetraglycine peptide (Gly-Gly-Gly-Gly) was studied by electrospray ionization mass spectrometry. Experiments were performed under alkaline conditions, in the presence of ethanolamine (pH 10.95). We observed that the presence of copper(II) ions induces the aggregation of the peptide and the formation of copper-bound complexes with higher molecular mass is favored, such as the oligomer complexes 3M+2Cu-3H and 4M+3Cu-5H. At 1:1 peptide-copper(II) ion ratio, the singly charged 3M+2Cu-3H oligomer complex is the base peak in the mass spectrum. Metal ion-induced oligomerization of neurotoxic peptides is well known in the literature; however, there are very few examples in which such oligomerization was directly observed by mass spectrometry. Our results show that application of short peptides can be useful to study the mechanism of metal ion binding and metal ion-induced oligomerization of peptides.
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540 Chemie
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alzheimer a-beta, mass-spectrometry, prion-protein, electrospray-ionization, binding properties, zinc, complexes, coordination, aggregation
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SCHLOSSER, Gitta, Raluca STEFANESCU, Michael PRZYBYLSKI, Manuela MURARIU, Ferenc HUDECZ, Gabi DROCHIOIU, 2007. Copper-induced oligomerization of peptides : a model study. In: European Journal of Mass Spectrometry. 2007, 13(5), pp. 331-337. ISSN 1356-1049. Available under: doi: 10.1255/ejms.889BibTex
@article{Schlosser2007Coppe-1017,
year={2007},
doi={10.1255/ejms.889},
title={Copper-induced oligomerization of peptides : a model study},
number={5},
volume={13},
issn={1356-1049},
journal={European Journal of Mass Spectrometry},
pages={331--337},
author={Schlosser, Gitta and Stefanescu, Raluca and Przybylski, Michael and Murariu, Manuela and Hudecz, Ferenc and Drochioiu, Gabi}
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<dcterms:abstract xml:lang="eng">In this work, copper-binding of the tetraglycine peptide (Gly-Gly-Gly-Gly) was studied by electrospray ionization mass spectrometry. Experiments were performed under alkaline conditions, in the presence of ethanolamine (pH 10.95). We observed that the presence of copper(II) ions induces the aggregation of the peptide and the formation of copper-bound complexes with higher molecular mass is favored, such as the oligomer complexes [3M+2Cu-3H](+) and [4M+3Cu-5H](+). At 1:1 peptide-copper(II) ion ratio, the singly charged [3M+2Cu-3H](+) oligomer complex is the base peak in the mass spectrum. Metal ion-induced oligomerization of neurotoxic peptides is well known in the literature; however, there are very few examples in which such oligomerization was directly observed by mass spectrometry. Our results show that application of short peptides can be useful to study the mechanism of metal ion binding and metal ion-induced oligomerization of peptides.</dcterms:abstract>
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