Publikation:

A conserved structural motif for lipopolysaccharide recognition by procaryotic and eucaryotic proteins

Lade...
Vorschaubild

Datum

2000

Autor:innen

Ferguson, Andrew D.
Hofmann, Eckhard
Lindner, Buko
Holst, Otto
Coulton, James W.

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

ArXiv-ID

Internationale Patentnummer

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung
Open Access Green
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

Structure. 2000, 8(6), pp. 585-592. ISSN 0969-2126. Available under: doi: 10.1016/S0969-2126(00)00143-X

Zusammenfassung

Background: Lipopolysaccharide (LPS), a lipoglycan from the outer membrane of Gram-negative bacteria, is an immunomodulatory molecule that stimulates the innate immune response. High levels of LPS cause excessive release of inflammatory mediators and are responsible for the septic shock syndrome. The interaction of LPS with its cognate binding proteins has not, as yet, been structurally elucidated.

Results: The X-ray crystallographic structure of LPS in complex with the integralouter membrane protein FhuA from Escherichia coli K-12 is reported. It is in accord with data obtained using mass spectroscopy and nuclear magnetic resonance. Most of the important hydrogen-bonding or electrostatic interactions with LPS are provided by eight positively charged residues of FhuA. Residues in a similar three-dimensional arrangement were searched for in all structurally known proteins using a fast template-matching algorithm, and a subset of four residues was identified that is common to known LPS-binding proteins.

Conclusions: These four residues, three of which form specific interactions with lipid A, appear to provide the structural basis of pattern recognition in the innate immune response. Their arrangement can serve to identify LPS-binding sites on proteins known to interact with LPS, and could serve as a template for molecular modeling of a LPS scavenger designed to reduce the septic shock syndrome.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

innate immune response, lipopolysaccharide, septic shock, siderophoremediated iron acquisition, TonB-dependent receptor

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690FERGUSON, Andrew D., Wolfram WELTE, Eckhard HOFMANN, Buko LINDNER, Otto HOLST, James W. COULTON, Kay DIEDERICHS, 2000. A conserved structural motif for lipopolysaccharide recognition by procaryotic and eucaryotic proteins. In: Structure. 2000, 8(6), pp. 585-592. ISSN 0969-2126. Available under: doi: 10.1016/S0969-2126(00)00143-X
BibTex
@article{Ferguson2000conse-8098,
  year={2000},
  doi={10.1016/S0969-2126(00)00143-X},
  title={A conserved structural motif for lipopolysaccharide recognition by procaryotic and eucaryotic proteins},
  number={6},
  volume={8},
  issn={0969-2126},
  journal={Structure},
  pages={585--592},
  author={Ferguson, Andrew D. and Welte, Wolfram and Hofmann, Eckhard and Lindner, Buko and Holst, Otto and Coulton, James W. and Diederichs, Kay}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/8098">
    <dcterms:abstract xml:lang="eng">Background: Lipopolysaccharide (LPS), a lipoglycan from the outer membrane of Gram-negative bacteria, is an immunomodulatory molecule that stimulates the innate immune response. High levels of LPS cause excessive release of inflammatory mediators and are responsible for the septic shock syndrome. The interaction of LPS with its cognate binding proteins has not, as yet, been structurally elucidated.&lt;br /&gt;&lt;br /&gt;Results: The X-ray crystallographic structure of LPS in complex with the integralouter membrane protein FhuA from Escherichia coli K-12 is reported. It is in accord with data obtained using mass spectroscopy and nuclear magnetic resonance. Most of the important hydrogen-bonding or electrostatic interactions with LPS are provided by eight positively charged residues of FhuA. Residues in a similar three-dimensional arrangement were searched for in all structurally known proteins using a fast template-matching algorithm, and a subset of four residues was identified that is common to known LPS-binding proteins.&lt;br /&gt;&lt;br /&gt;Conclusions: These four residues, three of which form specific interactions with lipid A, appear to provide the structural basis of pattern recognition in the innate immune response. Their arrangement can serve to identify LPS-binding sites on proteins known to interact with LPS, and could serve as a template for molecular modeling of a LPS scavenger designed to reduce the septic shock syndrome.</dcterms:abstract>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8098/1/A_conserved_structural_motif_for_lipopolysaccharide_recognition_by_procaryotic_and_eucaryotic_proteins.pdf"/>
    <dc:contributor>Holst, Otto</dc:contributor>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8098"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Diederichs, Kay</dc:contributor>
    <dcterms:title>A conserved structural motif for lipopolysaccharide recognition by procaryotic and eucaryotic proteins</dcterms:title>
    <dc:creator>Ferguson, Andrew D.</dc:creator>
    <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights>
    <dc:contributor>Lindner, Buko</dc:contributor>
    <dc:language>eng</dc:language>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8098/1/A_conserved_structural_motif_for_lipopolysaccharide_recognition_by_procaryotic_and_eucaryotic_proteins.pdf"/>
    <dc:creator>Hofmann, Eckhard</dc:creator>
    <dc:contributor>Coulton, James W.</dc:contributor>
    <dc:creator>Coulton, James W.</dc:creator>
    <dc:contributor>Welte, Wolfram</dc:contributor>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:39:55Z</dc:date>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:39:55Z</dcterms:available>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/>
    <dc:format>application/pdf</dc:format>
    <dc:contributor>Ferguson, Andrew D.</dc:contributor>
    <dc:contributor>Hofmann, Eckhard</dc:contributor>
    <dc:creator>Diederichs, Kay</dc:creator>
    <dcterms:issued>2000</dcterms:issued>
    <dc:creator>Holst, Otto</dc:creator>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:creator>Welte, Wolfram</dc:creator>
    <dcterms:bibliographicCitation>First publ. in: Structure 8 (2000), pp. 585-592</dcterms:bibliographicCitation>
    <dc:creator>Lindner, Buko</dc:creator>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
Begutachtet
Diese Publikation teilen