Publikation:

Efficient assays to quantify the life history traits of algal viruses

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Datum

2023

Autor:innen

Agarkova, Irina V.
Dunigan, David D.
Van Etten, James L.

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Open Access-Veröffentlichung
Open Access Hybrid
Core Facility der Universität Konstanz
Flow Cytometry Centre

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Applied and Environmental Microbiology. American Society for Microbiology. 2023, 89(12). ISSN 0099-2240. eISSN 1098-5336. Verfügbar unter: doi: 10.1128/aem.01659-23

Zusammenfassung

Although viral life history traits—the traits that determine reproductive and survival success—are critical to understanding the population dynamics and impact of viruses, they are understudied compared to molecular traits. This discrepancy is partly due to the challenge of phenotyping viral life histories. We developed improved methods to quantify the life cycles of chloroviruses, which are lytic double-stranded DNA viruses that infect unicellular “chlorella-like” algae. We modified classical one-step growth and survival assays by including flow cytometry and kinetic modeling, and applied these to four chlorovirus strains. Together, the modified assays quantified the full life cycle, including adsorption rate, probability of depolarizing the host cell, probability of releasing progeny virions, time until lysis, burst size, specific infectivity, and mortality rate of infectious virions. We also discovered that biphasic or “tailing” decay can occur in chloroviruses, and measured the fraction of infectious virions that resisted decay. The modified assays are more efficient than existing techniques, quantify more traits, and are applicable to other lytic viruses with quantifiable virions. The modified one-step growth assay is suitable for viruses with mutual exclusion, superinfection exclusion, or superinfection immunity; the modified survival assay can be applied to any lytic virus with quantifiable virions.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

lytic virus, phycodnavirus, flowflow virometry, one-step growth assay, survival assay, inactivation assay, kinetic models

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ISO 690LIEVENS, Eva J. P., Irina V. AGARKOVA, David D. DUNIGAN, James L. VAN ETTEN, Lutz BECKS, 2023. Efficient assays to quantify the life history traits of algal viruses. In: Applied and Environmental Microbiology. American Society for Microbiology. 2023, 89(12). ISSN 0099-2240. eISSN 1098-5336. Verfügbar unter: doi: 10.1128/aem.01659-23
BibTex
@article{Lievens2023-12-21Effic-68461,
  year={2023},
  doi={10.1128/aem.01659-23},
  title={Efficient assays to quantify the life history traits of algal viruses},
  number={12},
  volume={89},
  issn={0099-2240},
  journal={Applied and Environmental Microbiology},
  author={Lievens, Eva J. P. and Agarkova, Irina V. and Dunigan, David D. and Van Etten, James L. and Becks, Lutz}
}
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