Synthesis of Erythropoietins Site‐Specifically Conjugated with Complex‐Type N‐Glycans

Streichert, Katharina; Seitz, Carina; Hoffmann, Eugenia; Boos, Irene; Jelkmann, Wolfgang; Brunner, Thomas; Unverzagt, Carlo; Rubini, Marina

Synthesis of Erythropoietins Site‐Specifically Conjugated with Complex‐Type N‐Glycans

Dateien

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Datum

2019

Autor:innen

Streichert, Katharina

Seitz, Carina

Hoffmann, Eugenia

Boos, Irene

Jelkmann, Wolfgang

Brunner, Thomas

Unverzagt, Carlo

Rubini, Marina

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie
Chemie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

ChemBioChem. Wiley. 2019, 20(15), pp. 1914-1918. ISSN 1439-4227. eISSN 1439-7633. Available under: doi: 10.1002/cbic.201900023

Zusammenfassung

The biological activity of glycoprotein hormone erythropoietin (EPO) is dependent mainly on the structure of its N-linked glycans. We aimed at readily attaching N-glycans to EPO via defined alkyne groups. EPO variants with an alkyne bearing amino acid (Plk) at the N-glycosylation sites 24, 38 and 83 were obtained by amber suppression followed by refolding. Click-conjugation of the alkinyl EPOs with biantennary N-glycan azides provided biologically active site-specifically modified EPO glycoconjugates.

Fachgebiet (DDC)

540 Chemie

Schlagwörter

Click chemistry, Glycoproteins, Protein Engineering, Protein modifications, synthetic glycans

Zitieren

ISO 690

STREICHERT, Katharina, Carina SEITZ, Eugenia HOFFMANN, Irene BOOS, Wolfgang JELKMANN, Thomas BRUNNER, Carlo UNVERZAGT, Marina RUBINI, 2019. Synthesis of Erythropoietins Site‐Specifically Conjugated with Complex‐Type N‐Glycans. In: ChemBioChem. Wiley. 2019, 20(15), pp. 1914-1918. ISSN 1439-4227. eISSN 1439-7633. Available under: doi: 10.1002/cbic.201900023

BibTex

@article{Streichert2019-08-01Synth-46286,
  year={2019},
  doi={10.1002/cbic.201900023},
  title={Synthesis of Erythropoietins Site‐Specifically Conjugated with Complex‐Type N‐Glycans},
  number={15},
  volume={20},
  issn={1439-4227},
  journal={ChemBioChem},
  pages={1914--1918},
  author={Streichert, Katharina and Seitz, Carina and Hoffmann, Eugenia and Boos, Irene and Jelkmann, Wolfgang and Brunner, Thomas and Unverzagt, Carlo and Rubini, Marina}
}

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