Publikation: Effects of the ATP, ADP and inorganic phosphate on the transport rate of the Na,K-pump
Lade...
Dateien
Datum
1986
Autor:innen
Herausgeber:innen
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
URI (zitierfähiger Link)
DOI (zitierfähiger Link)
Internationale Patentnummer
Link zur Lizenz
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Open Access Green
Sammlungen
Core Facility der Universität Konstanz
Titel in einer weiteren Sprache
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
Biochimica et Biophysica Acta - Biomembranes. 1986, 857(1), pp. 105-115. ISSN 0005-2736. Available under: doi: 10.1016/0005-2736(86)90103-3
Zusammenfassung
(Na+K)-ATPase from kidney outer medulla was incorporated into artificial dioleoylphosphatidylcholine vesicles. In the reconstituted system the pump can be activated by adding ATP to the external medium. ATP-driven potassium extrusion by the Na,K-pump was studied using a voltage-sensitive dye in the presence of valinomycin. ADP strongly reduced the turnover rate of the pump with a concentration for half-maximal inhibition of cD,1/2 =0.1 mM. cD,1/2 was found to be virtually independent of ATP concentration, indicating that the inhibition is non-competitive with respect to ATP. The non-competitive inhibition by ADP can be explained on the basis of the Post-Albers reaction cycle of the Na,K-pump, assuming that the main action of ADP is the reversal of the phosphorylation step.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
(Na, K)-ATPase, Membrane reconstitution, Product inhibition, Kinetics, Fluorescence, (Rabbit kidney)
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690
APELL, Hans-Jürgen, M. NELSON, Madeleine M. MARCUS, Peter LÄUGER, 1986. Effects of the ATP, ADP and inorganic phosphate on the transport rate of the Na,K-pump. In: Biochimica et Biophysica Acta - Biomembranes. 1986, 857(1), pp. 105-115. ISSN 0005-2736. Available under: doi: 10.1016/0005-2736(86)90103-3BibTex
@article{Apell1986Effec-7113,
year={1986},
doi={10.1016/0005-2736(86)90103-3},
title={Effects of the ATP, ADP and inorganic phosphate on the transport rate of the Na,K-pump},
number={1},
volume={857},
issn={0005-2736},
journal={Biochimica et Biophysica Acta - Biomembranes},
pages={105--115},
author={Apell, Hans-Jürgen and Nelson, M. and Marcus, Madeleine M. and Läuger, Peter}
}RDF
<rdf:RDF
xmlns:dcterms="http://purl.org/dc/terms/"
xmlns:dc="http://purl.org/dc/elements/1.1/"
xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
xmlns:bibo="http://purl.org/ontology/bibo/"
xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
xmlns:foaf="http://xmlns.com/foaf/0.1/"
xmlns:void="http://rdfs.org/ns/void#"
xmlns:xsd="http://www.w3.org/2001/XMLSchema#" >
<rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/7113">
<dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:31:34Z</dcterms:available>
<dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights>
<dc:contributor>Läuger, Peter</dc:contributor>
<dc:creator>Nelson, M.</dc:creator>
<dc:contributor>Apell, Hans-Jürgen</dc:contributor>
<bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7113"/>
<dcterms:issued>1986</dcterms:issued>
<void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
<dc:creator>Marcus, Madeleine M.</dc:creator>
<dcterms:bibliographicCitation>First publ. in: Biochimica et Biophysica Acta - Biomembranes 857 (1986), 105-115</dcterms:bibliographicCitation>
<foaf:homepage rdf:resource="http://localhost:8080/"/>
<dcterms:title>Effects of the ATP, ADP and inorganic phosphate on the transport rate of the Na,K-pump</dcterms:title>
<dc:contributor>Marcus, Madeleine M.</dc:contributor>
<dc:language>eng</dc:language>
<dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7113/1/Effects_of_ATPADP_and_inorganic_phosphate.pdf"/>
<dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/>
<dc:contributor>Nelson, M.</dc:contributor>
<dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
<dc:creator>Läuger, Peter</dc:creator>
<dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
<dc:format>application/pdf</dc:format>
<dc:creator>Apell, Hans-Jürgen</dc:creator>
<dcterms:abstract xml:lang="eng">(Na+K)-ATPase from kidney outer medulla was incorporated into artificial dioleoylphosphatidylcholine vesicles. In the reconstituted system the pump can be activated by adding ATP to the external medium. ATP-driven potassium extrusion by the Na,K-pump was studied using a voltage-sensitive dye in the presence of valinomycin. ADP strongly reduced the turnover rate of the pump with a concentration for half-maximal inhibition of cD,1/2 =0.1 mM. cD,1/2 was found to be virtually independent of ATP concentration, indicating that the inhibition is non-competitive with respect to ATP. The non-competitive inhibition by ADP can be explained on the basis of the Post-Albers reaction cycle of the Na,K-pump, assuming that the main action of ADP is the reversal of the phosphorylation step.</dcterms:abstract>
<dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7113/1/Effects_of_ATPADP_and_inorganic_phosphate.pdf"/>
<dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:31:34Z</dc:date>
</rdf:Description>
</rdf:RDF>Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
