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Measuring the Enzyme Activity of Arabidopsis Deubiquitylating Enzymes

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2016

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Kalinowska, Kamila

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https://doi.org/10.1007/978-1-4939-3759-2_4
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LOIS, L. Maria, ed., Rune MATTHIESEN, ed.. Plant Proteostasis : Methods and Protocols. New York, NY: Humana Press, 2016, pp. 35-44. Methods in Molecular Biology. 1450. ISBN 978-1-4939-3757-8. Available under: doi: 10.1007/978-1-4939-3759-2_4

Zusammenfassung

Deubiquitylating enzymes, or DUBs, are important regulators of ubiquitin homeostasis and substrate stability, though the molecular mechanisms of most of the DUBs in plants are not yet understood. As different ubiquitin chain types are implicated in different biological pathways, it is important to analyze the enzyme characteristic for studying a DUB. Quantitative analysis of DUB activity is also important to determine enzyme kinetics and the influence of DUB binding proteins on the enzyme activity. Here, we show methods to analyze DUB activity using immunodetection, Coomassie Brilliant Blue staining, and fluorescence measurement that can be useful for understanding the basic characteristic of DUBs.

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570 Biowissenschaften, Biologie

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ISO 690KALINOWSKA, Kamila, Marie-Kristin NAGEL, Erika ISONO, 2016. Measuring the Enzyme Activity of Arabidopsis Deubiquitylating Enzymes. In: LOIS, L. Maria, ed., Rune MATTHIESEN, ed.. Plant Proteostasis : Methods and Protocols. New York, NY: Humana Press, 2016, pp. 35-44. Methods in Molecular Biology. 1450. ISBN 978-1-4939-3757-8. Available under: doi: 10.1007/978-1-4939-3759-2_4
BibTex
@incollection{Kalinowska2016-07-18Measu-39859,
  year={2016},
  doi={10.1007/978-1-4939-3759-2_4},
  title={Measuring the Enzyme Activity of Arabidopsis Deubiquitylating Enzymes},
  number={1450},
  number={1450},
  isbn={978-1-4939-3757-8},
  publisher={Humana Press},
  address={New York, NY},
  series={Methods in Molecular Biology},
  series={Methods in Molecular Biology},
  booktitle={Plant Proteostasis : Methods and Protocols},
  pages={35--44},
  editor={Lois, L. Maria and Matthiesen, Rune},
  author={Kalinowska, Kamila and Nagel, Marie-Kristin and Isono, Erika}
}
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