Publikation:

Development of a monoclonal antibody for ELISA of CYP1A in primary cultures of rainbow trout (Oncorhynchus mykiss) hepatocytes

Lade...
Vorschaubild

Datum

1997

Autor:innen

Scholz, Stefan
Behn, Ingrid
Honeck, Horst
Braunbeck, Thomas
Segner, Helmut

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

ArXiv-ID

Internationale Patentnummer

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung
Open Access Green
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

Biomarkers. 1997, 2(5), pp. 287-294. ISSN 1354-750X. eISSN 1366-5804. Available under: doi: 10.1080/135475097231517

Zusammenfassung

Induction of cytochrome P4501A CYP1A in cultured cells can be used to determine the induction potencies of xenobiotics or complex environmental samples. This report describes the development of an enzyme linked immunosorbent assay ELISA for measurement of CYP1A expression in primary cultures of rainbow trout Oncorhynchus mykiss hepatocytes. Juvenile rainbow trout were injected with naphthoflavone BNF 25 mg kg-1 body weight to induce the synthesis of CYP1A. The CYP1A isoenzyme was purified, characterized by immunological cross reactivity and N terminal sequencing and used to prepare a monoclonal antibody in Balb C mice. The specificity of the antibody for CYP1A was proved by Western blotting of samples from control and BNF injected fish. Two ELISA methods, a direct and a competitive one, were evaluated, with both methods being of comparable sensitivity. Rainbow trout hepatocytes, maintained as monolayers in serum free, chemically defined medium, were exposed to naphthoflavone, and the induction response was measured both by 7 ethoxyresorufin O deethylase EROD activity and the direct ELISA method. Comparison between EROD activity and immunodetectable CYP1A protein can provide information on the catalytic efficiency of CYP1A.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Cytochrome P4501a, Primary Culture Induction, Elisa, Monoclonal Antibody

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690SCHOLZ, Stefan, Ingrid BEHN, Horst HONECK, Christof R. HAUCK, Thomas BRAUNBECK, Helmut SEGNER, 1997. Development of a monoclonal antibody for ELISA of CYP1A in primary cultures of rainbow trout (Oncorhynchus mykiss) hepatocytes. In: Biomarkers. 1997, 2(5), pp. 287-294. ISSN 1354-750X. eISSN 1366-5804. Available under: doi: 10.1080/135475097231517
BibTex
@article{Scholz1997Devel-6903,
  year={1997},
  doi={10.1080/135475097231517},
  title={Development of a monoclonal antibody for ELISA of CYP1A in primary cultures of rainbow trout (Oncorhynchus mykiss) hepatocytes},
  number={5},
  volume={2},
  issn={1354-750X},
  journal={Biomarkers},
  pages={287--294},
  author={Scholz, Stefan and Behn, Ingrid and Honeck, Horst and Hauck, Christof R. and Braunbeck, Thomas and Segner, Helmut}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/6903">
    <dcterms:abstract xml:lang="eng">Induction of cytochrome P4501A CYP1A in cultured cells can be used to determine the induction potencies of xenobiotics or complex environmental samples. This report describes the development of an enzyme linked immunosorbent assay ELISA for measurement of CYP1A expression in primary cultures of rainbow trout Oncorhynchus mykiss hepatocytes. Juvenile rainbow trout were injected with naphthoflavone BNF 25 mg kg-1 body weight to induce the synthesis of CYP1A. The CYP1A isoenzyme was purified, characterized by immunological cross reactivity and N terminal sequencing and used to prepare a monoclonal antibody in Balb C mice. The specificity of the antibody for CYP1A was proved by Western blotting of samples from control and BNF injected fish. Two ELISA methods, a direct and a competitive one, were evaluated, with both methods being of comparable sensitivity. Rainbow trout hepatocytes, maintained as monolayers in serum free, chemically defined medium, were exposed to naphthoflavone, and the induction response was measured both by 7 ethoxyresorufin O deethylase EROD activity and the direct ELISA method. Comparison between EROD activity and immunodetectable CYP1A protein can provide information on the catalytic efficiency of CYP1A.</dcterms:abstract>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/6903/1/Development_of_a_monoclonal_antibody_for_ELISA_of_CYP1A_in_primary_cultures_of_rainbow_trout.PDF"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:bibliographicCitation>First publ. in: Biomarkers 2 (1997), pp. 287-294</dcterms:bibliographicCitation>
    <dc:contributor>Hauck, Christof R.</dc:contributor>
    <dc:format>application/pdf</dc:format>
    <dc:creator>Behn, Ingrid</dc:creator>
    <dc:contributor>Braunbeck, Thomas</dc:contributor>
    <dc:creator>Segner, Helmut</dc:creator>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/>
    <dc:language>eng</dc:language>
    <dcterms:issued>1997</dcterms:issued>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:30:02Z</dc:date>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:30:02Z</dcterms:available>
    <dc:contributor>Honeck, Horst</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights>
    <dc:creator>Scholz, Stefan</dc:creator>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:contributor>Behn, Ingrid</dc:contributor>
    <dc:creator>Braunbeck, Thomas</dc:creator>
    <dc:contributor>Segner, Helmut</dc:contributor>
    <dcterms:title>Development of a monoclonal antibody for ELISA of CYP1A in primary cultures of rainbow trout (Oncorhynchus mykiss) hepatocytes</dcterms:title>
    <dc:creator>Honeck, Horst</dc:creator>
    <dc:creator>Hauck, Christof R.</dc:creator>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/6903/1/Development_of_a_monoclonal_antibody_for_ELISA_of_CYP1A_in_primary_cultures_of_rainbow_trout.PDF"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Scholz, Stefan</dc:contributor>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/6903"/>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
Begutachtet
Diese Publikation teilen