Publikation:

Efficient labelling of enzymatically synthesized vinyl-modified DNA by an inverse-electron-demand Diels-Alder reaction

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Busskamp_286929.pdf
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2014

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http://nbn-resolving.de/urn:nbn:de:bsz:352-286929
DOI (zitierfähiger Link)
https://doi.org/10.1039/C4CC04332D
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Chemie: Publikationen
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Chemical Communications. 2014, 50(74), pp. 10827-10829. ISSN 1359-7345. eISSN 1364-548X. Available under: doi: 10.1039/C4CC04332D

Zusammenfassung

Many applications in biotechnology and molecular biology rely on modified nucleotides. Here, we present an approach for the postsynthetic labelling of enzymatically synthesized vinyl-modified DNA by Diels–Alder reaction with inverse electron demand using a tetrazine. Labelling proceeds very efficiently and supersedes several known approaches.

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540 Chemie

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ISO 690BUSSKAMP, Holger, Ellen BATROFF, Andrea NIEDERWIESER, Obadah S. ABDEL-RAHMAN, Rainer F. WINTER, Valentin WITTMANN, Andreas MARX, 2014. Efficient labelling of enzymatically synthesized vinyl-modified DNA by an inverse-electron-demand Diels-Alder reaction. In: Chemical Communications. 2014, 50(74), pp. 10827-10829. ISSN 1359-7345. eISSN 1364-548X. Available under: doi: 10.1039/C4CC04332D
BibTex
@article{Bukamp2014-09-25Effic-28692,
  year={2014},
  doi={10.1039/C4CC04332D},
  title={Efficient labelling of enzymatically synthesized vinyl-modified DNA by an inverse-electron-demand Diels-Alder reaction},
  number={74},
  volume={50},
  issn={1359-7345},
  journal={Chemical Communications},
  pages={10827--10829},
  author={Bußkamp, Holger and Batroff, Ellen and Niederwieser, Andrea and Abdel-Rahman, Obadah S. and Winter, Rainer F. and Wittmann, Valentin and Marx, Andreas}
}
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