Publikation: 1H-NMR and mass spectrometric studies of tetrahydropterins : evidence for the structure of 6-pyruvoyl tetrahydropterin, an intermediate in the biosynthesis of tetrahydrobiopterin
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The conversion of dihydroneopterin triphosphate in the presence of 6-pyruvoyl tetrahydropterin synthase was followed by 1H-NMR spectroscopy. The interpretation of the spectra of the product is unequivocal: they show formation of a tetrahydropterin system carrying a stereospecifically oriented substituent at the asymmetric C(6) atom. The spectra are compatible with formation of a (3')-CH3 function, and with complete removal of the 1' and 2' hydrogens of dihydroneopterin triphosphate. The fast-atom-bombardment/mass spectrometry study of the same product yields a [M + H]+ ion at m/z 238 compatible with the structure of 6-pyruvoyl tetrahydropterin. The data support the proposed structure of 6-pyruvoyl tetrahydropterin as a key intermediate in the biosynthesis of tetrahydrobiopterin.
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GHISLA, Sandro, Thomas KUSTER, Petra STEINERSTAUCH, Walter LEIMBACHER, Wilhelm Johannes RICHTER, Fritz RASCHDORF, Roland DAHINDEN, Hans-Christoph CURTIUS, 1990. 1H-NMR and mass spectrometric studies of tetrahydropterins : evidence for the structure of 6-pyruvoyl tetrahydropterin, an intermediate in the biosynthesis of tetrahydrobiopterin. In: European Journal of Biochemistry. 1990, 187(3), pp. 651-656. ISSN 0014-2956. eISSN 1432-1033. Available under: doi: 10.1111/j.1432-1033.1990.tb15349.xBibTex
@article{Ghisla19901HNMR-6578, year={1990}, doi={10.1111/j.1432-1033.1990.tb15349.x}, title={1H-NMR and mass spectrometric studies of tetrahydropterins : evidence for the structure of 6-pyruvoyl tetrahydropterin, an intermediate in the biosynthesis of tetrahydrobiopterin}, number={3}, volume={187}, issn={0014-2956}, journal={European Journal of Biochemistry}, pages={651--656}, author={Ghisla, Sandro and Kuster, Thomas and Steinerstauch, Petra and Leimbacher, Walter and Richter, Wilhelm Johannes and Raschdorf, Fritz and Dahinden, Roland and Curtius, Hans-Christoph} }
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