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Structured light sheet fluorescence microscopy based on four beam interference

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2010

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Optics Express : the international electronic journal of optics. 2010, 18(18), pp. 19232-19241. eISSN 1094-4087. Available under: doi: 10.1364/OE.18.019232

Zusammenfassung

A 3D structured light sheet microscope using a four-faceted symmetric pyramid is presented. The sample is illuminated by the resulting four beam interference field. This approach combines advantages of standing wave and structured illumination microscopy. Examples of micrographs of fluorescently labeled Chinese hamster ovary (CHO) cells as well as of the compound eyes of drosophila are shown and the optical sectioning ability of our system is demonstrated. The capabilities and the limitations of the scheme are discussed.

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540 Chemie

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ISO 690LEI, Ming, Andreas ZUMBUSCH, 2010. Structured light sheet fluorescence microscopy based on four beam interference. In: Optics Express : the international electronic journal of optics. 2010, 18(18), pp. 19232-19241. eISSN 1094-4087. Available under: doi: 10.1364/OE.18.019232
BibTex
@article{Lei2010Struc-9965,
  year={2010},
  doi={10.1364/OE.18.019232},
  title={Structured light sheet fluorescence microscopy based on four beam interference},
  number={18},
  volume={18},
  journal={Optics Express : the international electronic journal of optics},
  pages={19232--19241},
  author={Lei, Ming and Zumbusch, Andreas}
}
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