Publikation: Identification of astrocyte- and oligodendrocyte-like cells of goldfish optic nerves in culture
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1989
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Neuroscience Letters. 1989, 101(2), pp. 127-132. ISSN 0304-3940. Available under: doi: 10.1016/0304-3940(89)90518-1
Zusammenfassung
Fish glial cells were obtained from cultivated segments of the optic nerve and raised in vitro. Two types of cells were identified as astrocyte- and oligodendrocyte-like glia by the monoclonal antibody Mab O1 (specific for oligodendrocytes) and the rabbit serum anti-goldfish glial fibrillary acidic protein (anti-G-GFAP). Cells of compact morphology were rare, and anti-G-GFAP positive and O1 negative. Multipolar cells in 5-day-old cultures were anti-G-GFAP but rarely O1 positive. In 5-week-old cultures, however, roughly 75% of the multipolar cells were double-labeled with both anti-G-GFAP and O1; 10% were single labeled with Mab O1 and 15% with anti-G-GFAP, respectively.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
Glial cell, Goldfish optic nerve, In culture, Immunostaining
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BASTMEYER, Martin, Matthias BECKMANN, Shmaiel N. NONA, John R. CRONLY-DILLON, Claudia STÜRMER, 1989. Identification of astrocyte- and oligodendrocyte-like cells of goldfish optic nerves in culture. In: Neuroscience Letters. 1989, 101(2), pp. 127-132. ISSN 0304-3940. Available under: doi: 10.1016/0304-3940(89)90518-1BibTex
@article{Bastmeyer1989Ident-6629,
year={1989},
doi={10.1016/0304-3940(89)90518-1},
title={Identification of astrocyte- and oligodendrocyte-like cells of goldfish optic nerves in culture},
number={2},
volume={101},
issn={0304-3940},
journal={Neuroscience Letters},
pages={127--132},
author={Bastmeyer, Martin and Beckmann, Matthias and Nona, Shmaiel N. and Cronly-Dillon, John R. and Stürmer, Claudia}
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<dcterms:abstract xml:lang="deu">Fish glial cells were obtained from cultivated segments of the optic nerve and raised in vitro. Two types of cells were identified as astrocyte- and oligodendrocyte-like glia by the monoclonal antibody Mab O1 (specific for oligodendrocytes) and the rabbit serum anti-goldfish glial fibrillary acidic protein (anti-G-GFAP). Cells of compact morphology were rare, and anti-G-GFAP positive and O1 negative. Multipolar cells in 5-day-old cultures were anti-G-GFAP but rarely O1 positive. In 5-week-old cultures, however, roughly 75% of the multipolar cells were double-labeled with both anti-G-GFAP and O1; 10% were single labeled with Mab O1 and 15% with anti-G-GFAP, respectively.</dcterms:abstract>
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