@article{Ladenburger2006Ins14-9695,
  year={2006},
  doi={10.1242/jcs.03075},
  title={An Ins(1,4,5)P3 receptor in Paramecium is associatedwith the osmoregulatory system},
  number={17},
  volume={119},
  issn={0021-9533},
  journal={Journal of Cell Science},
  pages={3705--3717},
  author={Ladenburger, Eva-Maria and Korn, Iris and Kasielke, Nicole and Wassmer, Thomas and Plattner, Helmut}
}

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    <dcterms:bibliographicCitation>First publ. in: Journal of Cell Science 119 (2006), pp. 3705-3717</dcterms:bibliographicCitation>
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    <dc:creator>Wassmer, Thomas</dc:creator>
    <dc:contributor>Kasielke, Nicole</dc:contributor>
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    <dc:creator>Kasielke, Nicole</dc:creator>
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    <dc:language>eng</dc:language>
    <dcterms:issued>2006</dcterms:issued>
    <dc:contributor>Plattner, Helmut</dc:contributor>
    <dc:contributor>Wassmer, Thomas</dc:contributor>
    <dcterms:title>An Ins(1,4,5)P3 receptor in Paramecium is associatedwith the osmoregulatory system</dcterms:title>
    <dc:creator>Plattner, Helmut</dc:creator>
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    <dc:contributor>Ladenburger, Eva-Maria</dc:contributor>
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    <dcterms:abstract xml:lang="eng">In the ciliate Paramecium, a variety of well characterized processes are regulated by Ca2+, e.g. exocytosis, endocytosis and ciliary beat. Therefore, among protozoa, Paramecium is considered a model organism for Ca2+ signaling, although the molecular identity of the channels responsible for the Ca2+ signals remains largely unknown. We have cloned   for the first time in a protozoan   the full sequence of the gene encoding a putative inositol (1,4,5)-trisphosphate (Ins(1,4,5)P3) receptor from Paramecium tetraurelia cells showing molecular characteristics of higher eukaryotic cells. The homologously expressed Ins(1,4,5)P3-binding domain binds [3H]Ins(1,4,5)P3, whereas antibodies unexpectedly localize this protein to the osmoregulatorysystem. The level of Ins(1,4,5)P3-receptor expression was reduced, as shown on a transcriptional level and by immuno-staining, by decreasing the concentration of extracellular Ca2+ (Paramecium cells rapidly adjust their Ca2+ level to that in the outside medium). Fluorochromes reveal spontaneous fluctuations in cytosolic Ca2+ levels along the osmoregulatory system and these signals change upon activation of caged Ins(1,4,5)P3. Considering the ongoing expulsion of substantial amounts of Ca2+ by the osmoregulatory system, we propose here that Ins(1,4,5)P3 receptors serve a new function, i.e. a latent, graded reflux of Ca2+ to fine-tune [Ca2+] homeostasis.</dcterms:abstract>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T18:13:46Z</dc:date>
    <dc:creator>Korn, Iris</dc:creator>
    <dc:creator>Ladenburger, Eva-Maria</dc:creator>
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