Simultaneous Detection of 14 Microcystin Congeners from Tissue Samples Using UPLC- ESI-MS/MS and Two Different Deuterated Synthetic Microcystins as Internal Standards

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Toxins. 2019, 11(7), 388. eISSN 2072-6651. Available under: doi: 10.3390/toxins11070388
Zusammenfassung

Cyanobacterial microcystins (MCs), potent serine/threonine-phosphatase inhibitors, pose an increasing threat to humans. Current detection methods are optimised for water matrices with only a few MC congeners simultaneously detected. However, as MC congeners are known to differ in their toxicity, methods are needed that simultaneously quantify the congeners present, thus allowing for summary hazard and risk assessment. Moreover, detection of MCs should be expanded to complex matrices, e.g., blood and tissue samples, to verify in situ MC concentrations, thus providing for improved exposure assessment and hazard interpretation. To achieve this, we applied two synthetic deuterated MC standards and optimised the tissue extraction protocol for the simultaneous detection of 14 MC congeners in a single ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) run. This procedure was validated using plasma and liver homogenates of mice (male and female) spiked with deuterated MC standards. For proof of concept, tissue and plasma samples from mice i.p. injected with MC-LR and MC-LF were analysed. While MC-LF was detected in all tissue samples of both sexes, detection of MC-LR was restricted to liver samples of male mice, suggesting different toxicokinetics in males, e.g., transport, conjugation or protein binding. Thus, deconjugation/-proteinisation steps should be employed to improve detection of bound MC.

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Fachgebiet (DDC)
540 Chemie
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cyanobacterial toxin; deuterated MC standards; microcystin; blood; liver tissue; UPLC-MS/MS; quantification
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ISO 690ALTANER, Stefan, Jonathan PUDDICK, Valerie FESSARD, Daniel FEURSTEIN, Ivan ZEMSKOV, Valentin WITTMANN, Daniel R. DIETRICH, 2019. Simultaneous Detection of 14 Microcystin Congeners from Tissue Samples Using UPLC- ESI-MS/MS and Two Different Deuterated Synthetic Microcystins as Internal Standards. In: Toxins. 2019, 11(7), 388. eISSN 2072-6651. Available under: doi: 10.3390/toxins11070388
BibTex
@article{Altaner2019-07-02Simul-46498,
  year={2019},
  doi={10.3390/toxins11070388},
  title={Simultaneous Detection of 14 Microcystin Congeners from Tissue Samples Using UPLC- ESI-MS/MS and Two Different Deuterated Synthetic Microcystins as Internal Standards},
  number={7},
  volume={11},
  journal={Toxins},
  author={Altaner, Stefan and Puddick, Jonathan and Fessard, Valerie and Feurstein, Daniel and Zemskov, Ivan and Wittmann, Valentin and Dietrich, Daniel R.},
  note={Article Number: 388}
}
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