Enzymes involved in phthalate degradation in sulphate-reducing bacteria

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2019
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Geiger, Robin Alexander
Mergelsberg, Mario
Ebenau-Jehle, Christa
Jesenofsky, Vivien Jill
Jehmlich, Nico
von Bergen, Martin
Boll, Matthias
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Environmental Microbiology. 2019, 21(10), pp. 3601-3612. ISSN 1462-2912. eISSN 1462-2920. Available under: doi: 10.1111/1462-2920.14681
Zusammenfassung

The complete degradation of the xenobiotic and environmentally harmful phthalate esters is initiated by hydrolysis to alcohols and o-phthalate (phthalate) by esterases. While further catabolism of phthalate has been studied in aerobic and denitrifying microorganisms, the degradation in obligately anaerobic bacteria has remained obscure. Here, we demonstrate a previously overseen growth of the δ-proteobacterium Desulfosarcina cetonica with phthalate/sulphate as only carbon and energy sources. Differential proteome and CoA ester pool analyses together with in vitro enzyme assays identified the genes, enzymes and metabolites involved in phthalate uptake and degradation in D. cetonica. Phthalate is initially activated to the short-lived phthaloyl-CoA by an ATP-dependent phthalate CoA ligase (PCL) followed by decarboxylation to the central intermediate benzoyl-CoA by an UbiD-like phthaloyl-CoA decarboxylase (PCD) containing a prenylated flavin cofactor. Genome/metagenome analyses predicted phthalate degradation capacity also in the sulphate-reducing Desulfobacula toluolica, strain NaphS2, and other δ-proteobacteria. Our results suggest that phthalate degradation proceeds in all anaerobic bacteria via the labile phthaloyl-CoA that is captured and decarboxylated by highly abundant PCDs. In contrast, two alternative strategies have been established for the formation of phthaloyl-CoA, the possibly most unstable CoA ester in biology.

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ISO 690GEIGER, Robin Alexander, Madan JUNGHARE, Mario MERGELSBERG, Christa EBENAU-JEHLE, Vivien Jill JESENOFSKY, Nico JEHMLICH, Martin VON BERGEN, Bernhard SCHINK, Matthias BOLL, 2019. Enzymes involved in phthalate degradation in sulphate-reducing bacteria. In: Environmental Microbiology. 2019, 21(10), pp. 3601-3612. ISSN 1462-2912. eISSN 1462-2920. Available under: doi: 10.1111/1462-2920.14681
BibTex
@article{Geiger2019-10Enzym-46558,
  year={2019},
  doi={10.1111/1462-2920.14681},
  title={Enzymes involved in phthalate degradation in sulphate-reducing bacteria},
  number={10},
  volume={21},
  issn={1462-2912},
  journal={Environmental Microbiology},
  pages={3601--3612},
  author={Geiger, Robin Alexander and Junghare, Madan and Mergelsberg, Mario and Ebenau-Jehle, Christa and Jesenofsky, Vivien Jill and Jehmlich, Nico and von Bergen, Martin and Schink, Bernhard and Boll, Matthias}
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