Publikation: Direct Sensing of 5-Methylcytosine by Polymerase Chain Reaction
Lade...
Dateien
Datum
2014
Herausgeber:innen
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
URI (zitierfähiger Link)
DOI (zitierfähiger Link)
Internationale Patentnummer
Link zur Lizenz
Angaben zur Forschungsförderung
European Union (EU): 339834
Projekt
EvoEPIGEN - Evolved Replication Systems of Epigenetics
Open Access-Veröffentlichung
Open Access Hybrid
Sammlungen
Core Facility der Universität Konstanz
Titel in einer weiteren Sprache
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
Angewandte Chemie International Edition. 2014, 53(31), pp. 8154-8158. ISSN 1433-7851. eISSN 1521-3773. Available under: doi: 10.1002/anie.201403745
Zusammenfassung
The epigenetic control of genes by the methylation of cytosine resulting in 5-methylcytosine (5mC) has fundamental implications for human development and disease. Analysis of alterations in DNA methylation patterns is an emerging tool for cancer diagnostics and prognostics. Here we report that two thermostable DNA polymerases, namely the DNA polymerase KlenTaq derived from Thermus aquaticus and the KOD DNA polymerase from Thermococcus kodakaraensis, are able to extend 3′-mismatched primer strands more efficiently from 5 mC than from unmethylated C. This feature was advanced by generating a DNA polymerase mutant with further improved 5mC/C discrimination properties and its successful application in a novel methylation-specific PCR approach directly from untreated human genomic DNA.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
540 Chemie
Schlagwörter
DNA methylation, DNA polymerases, enzyme engineering, polymerase chain reaction
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690
ASCHENBRENNER, Joos, Matthias DRUM, Hüsnü TOPAL, Markus WIELAND, Andreas MARX, 2014. Direct Sensing of 5-Methylcytosine by Polymerase Chain Reaction. In: Angewandte Chemie International Edition. 2014, 53(31), pp. 8154-8158. ISSN 1433-7851. eISSN 1521-3773. Available under: doi: 10.1002/anie.201403745BibTex
@article{Aschenbrenner2014-07-28Direc-28340,
year={2014},
doi={10.1002/anie.201403745},
title={Direct Sensing of 5-Methylcytosine by Polymerase Chain Reaction},
number={31},
volume={53},
issn={1433-7851},
journal={Angewandte Chemie International Edition},
pages={8154--8158},
author={Aschenbrenner, Joos and Drum, Matthias and Topal, Hüsnü and Wieland, Markus and Marx, Andreas}
}RDF
<rdf:RDF
xmlns:dcterms="http://purl.org/dc/terms/"
xmlns:dc="http://purl.org/dc/elements/1.1/"
xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
xmlns:bibo="http://purl.org/ontology/bibo/"
xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
xmlns:foaf="http://xmlns.com/foaf/0.1/"
xmlns:void="http://rdfs.org/ns/void#"
xmlns:xsd="http://www.w3.org/2001/XMLSchema#" >
<rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28340">
<dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/28340/2/Aschenbrenner_283401.pdf"/>
<dc:creator>Wieland, Markus</dc:creator>
<dc:contributor>Aschenbrenner, Joos</dc:contributor>
<dc:creator>Aschenbrenner, Joos</dc:creator>
<dcterms:issued>2014-07-28</dcterms:issued>
<foaf:homepage rdf:resource="http://localhost:8080/"/>
<dc:language>eng</dc:language>
<dc:creator>Marx, Andreas</dc:creator>
<dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2014-07-10T12:53:31Z</dc:date>
<dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/28340/2/Aschenbrenner_283401.pdf"/>
<dc:contributor>Marx, Andreas</dc:contributor>
<bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/28340"/>
<dcterms:bibliographicCitation>Angewandte Chemie : International Edition ; 53 (2014), 31. - S. 8154-8158</dcterms:bibliographicCitation>
<dc:rights>terms-of-use</dc:rights>
<dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
<dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
<dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
<dc:contributor>Topal, Hüsnü</dc:contributor>
<dc:creator>Topal, Hüsnü</dc:creator>
<dcterms:abstract xml:lang="eng">The epigenetic control of genes by the methylation of cytosine resulting in 5-methylcytosine (5mC) has fundamental implications for human development and disease. Analysis of alterations in DNA methylation patterns is an emerging tool for cancer diagnostics and prognostics. Here we report that two thermostable DNA polymerases, namely the DNA polymerase KlenTaq derived from Thermus aquaticus and the KOD DNA polymerase from Thermococcus kodakaraensis, are able to extend 3′-mismatched primer strands more efficiently from 5 mC than from unmethylated C. This feature was advanced by generating a DNA polymerase mutant with further improved 5mC/C discrimination properties and its successful application in a novel methylation-specific PCR approach directly from untreated human genomic DNA.</dcterms:abstract>
<dc:contributor>Drum, Matthias</dc:contributor>
<dcterms:title>Direct Sensing of 5-Methylcytosine by Polymerase Chain Reaction</dcterms:title>
<dc:contributor>Wieland, Markus</dc:contributor>
<dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2014-07-10T12:53:31Z</dcterms:available>
<dc:creator>Drum, Matthias</dc:creator>
<void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
</rdf:Description>
</rdf:RDF>Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
