Enzymatic Synthesis of Organic-Polymer-Grafted DNA
Enzymatic Synthesis of Organic-Polymer-Grafted DNA
Loading...
Date
2010
Authors
Editors
Journal ISSN
Electronic ISSN
ISBN
Bibliographical data
Publisher
Series
URI (citable link)
DOI (citable link)
International patent number
Link to the license
EU project number
Project
Open Access publication
Collections
Title in another language
Publication type
Journal article
Publication status
Published in
Chemistry : A European Journal ; 16 (2010), 1. - pp. 218-226. - ISSN 0947-6539. - eISSN 1521-3765
Abstract
To create bioorganic hybrid materials, interdisciplinary work in the fields of chemistry, biology and materials science is conducted. DNA block copolymers are promising hybrid materials due to the combination of properties intrinsic to both the polymer and the nucleic acid blocks. Until now, the coupling of DNA and organic polymers has been exercised post-synthetically in solution or on solid support. Herein, we report the first enzyme-catalysed synthesis of DNA organic polymer chimeras. For this purpose, four novel 2 -deoxyuridine triphosphates carrying polymer-like moieties linked to the nucleobase were synthesised. Linear polyethylene glycol monomethyl ethers of different sizes (1) and branched polyamido dendrons with varying terminal groups (2) were chosen as building blocks. We investigated the ability of DNA polymerases to accept the copolymers in comparison to the natural substrate and showed, through primer extensions, polymerase chain reactions and rolling circle amplification, that these building blocks could serve as a surrogate for the natural thymidine. By this method, DNA hybrid materials with high molecular weight, modification density, and defined structure are accessible.
Summary in another language
Subject (DDC)
540 Chemistry
Keywords
dendrimers,DNA copolymers,enzyme catalysis,nucleotides,polyethylene glycol
Conference
Review
undefined / . - undefined, undefined. - (undefined; undefined)
Cite This
ISO 690
BACCARO, Anna, Andreas MARX, 2010. Enzymatic Synthesis of Organic-Polymer-Grafted DNA. In: Chemistry : A European Journal. 16(1), pp. 218-226. ISSN 0947-6539. eISSN 1521-3765. Available under: doi: 10.1002/chem.200902296BibTex
@article{Baccaro2010Enzym-9905, year={2010}, doi={10.1002/chem.200902296}, title={Enzymatic Synthesis of Organic-Polymer-Grafted DNA}, number={1}, volume={16}, issn={0947-6539}, journal={Chemistry : A European Journal}, pages={218--226}, author={Baccaro, Anna and Marx, Andreas} }
RDF
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/9905"> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T18:15:13Z</dc:date> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/9905"/> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/9905/1/Baccaro_Chemistry_2010.pdf"/> <dc:language>eng</dc:language> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/> <dcterms:issued>2010</dcterms:issued> <dc:contributor>Baccaro, Anna</dc:contributor> <dc:creator>Marx, Andreas</dc:creator> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/> <dc:format>application/pdf</dc:format> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/9905/1/Baccaro_Chemistry_2010.pdf"/> <dc:contributor>Marx, Andreas</dc:contributor> <dcterms:bibliographicCitation>First publ. in: Chemistry : A European Journal 16 (2010), 1, pp. 218-226</dcterms:bibliographicCitation> <dc:rights>terms-of-use</dc:rights> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:title>Enzymatic Synthesis of Organic-Polymer-Grafted DNA</dcterms:title> <dcterms:abstract xml:lang="eng">To create bioorganic hybrid materials, interdisciplinary work in the fields of chemistry, biology and materials science is conducted. DNA block copolymers are promising hybrid materials due to the combination of properties intrinsic to both the polymer and the nucleic acid blocks. Until now, the coupling of DNA and organic polymers has been exercised post-synthetically in solution or on solid support. Herein, we report the first enzyme-catalysed synthesis of DNA organic polymer chimeras. For this purpose, four novel 2 -deoxyuridine triphosphates carrying polymer-like moieties linked to the nucleobase were synthesised. Linear polyethylene glycol monomethyl ethers of different sizes (1) and branched polyamido dendrons with varying terminal groups (2) were chosen as building blocks. We investigated the ability of DNA polymerases to accept the copolymers in comparison to the natural substrate and showed, through primer extensions, polymerase chain reactions and rolling circle amplification, that these building blocks could serve as a surrogate for the natural thymidine. By this method, DNA hybrid materials with high molecular weight, modification density, and defined structure are accessible.</dcterms:abstract> <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/> <foaf:homepage rdf:resource="http://localhost:8080/"/> <dc:creator>Baccaro, Anna</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T18:15:13Z</dcterms:available> </rdf:Description> </rdf:RDF>
Internal note
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Examination date of dissertation
Method of financing
Comment on publication
Alliance license
Corresponding Authors der Uni Konstanz vorhanden
International Co-Authors
Bibliography of Konstanz
Yes